PA Janelia Fluor^® 549, NHS ester

Key information: PA Janelia Fluor^® 549, NHS ester is a yellow photoactivatable fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live and fixed cell imaging.

Application: Suitable for single molecule tracking and super resolution microscopy (SRM) in live cells, specifically sptPALM (live cells) and PALM (fixed cells) techniques.

Properties and Photophysical Data: Non-fluorescent until activated at 405 nm. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag^® and SNAP-tag^®. Can be multiplexed with PA Janelia Fluor^® 646, NHS ester (Cat. No. 6150) to perform two-color sptPALM in live cells with activation at 405 nm. Photochemical quantum yield of uncaging = 2.2%, with improved yield upon protein conjugation. Excitation and emission maxima (λ) are 551 - 553 nm and 570 - 573 nm, respectively.

Please see the protocol for further information on protein/antibody labeling and conjugation.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.

This product has been recently renamed. The previous name for this product was PA Janelia Fluor^® 549, SE.

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus