PA Janelia Fluor^® 646, NHS ester

Key information: PA Janelia Fluor^® 646, NHS ester is a photoactivatable fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live and fixed cell imaging.

Application: Suitable for single molecule tracking and super resolution microscopy in live cells, specifically sptPALM (live cells) and PALM (fixed cells) techniques.

Properties and Photophysical Data: Non-fluorescent until activated at 405 nm. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag^® and SNAP-tag^®. Can be multiplexed with PA Janelia Fluor^® 549, NHS ester (Cat. No. 6149) to perform two-color sptPALM in live cells with activation at 405nm. Upon protein conjugation the efficiencies of photoconversion improve substantially. Low background staining. Excitation and emission maxima (λ) are 649 - 651 nm and 663 - 665 nm, respectively.

Please see the protocol for further information on protein/antibody labeling and conjugation.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

This product has been recently renamed. The previous name for this product was PA Janelia Fluor^® 646, SE.

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus