Sheep IgG F(ab)2 Isotype Control [FITC]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-96934
Forumulation
Catalog #
Key Product Details
Species Reactivity
Sheep
Applications
Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence
Label
FITC (Excitation = 495 nm, Emission = 519 nm)
Antibody Source
Sheep IgG
Concentration
LYOPH mg/ml
Product Specifications
Specificity
No reaction was observed against anti-Sheep IgG F(c) or anti-Pepsin.
Host
Sheep
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Sheep IgG, anti-Sheep IgG F(ab')2 and anti-Sheep Serum
This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Sheep IgG, anti-Sheep IgG F(ab')2 and anti-Sheep Serum
Applications for Sheep IgG F(ab)2 Isotype Control [FITC]
Application
Recommended Usage
Fluorophore-linked immunosorbent assay
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 1.0 ml deionized water (or equivalent).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgG F(ab)2
Additional IgG F(ab)2 Products
Product Documents for Sheep IgG F(ab)2 Isotype Control [FITC]
Product Specific Notices for Sheep IgG F(ab)2 Isotype Control [FITC]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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