Sheep IgG F(ab)2 Isotype Control
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97013
Conjugate
Catalog #
Key Product Details
Species Reactivity
Sheep
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Sheep IgG
Format
Purified
Concentration
Please contact technical services for concentration.
Product Summary for Sheep IgG F(ab)2 Isotype Control
Specificity
No reaction was observed against anti-Sheep IgG F(c) or anti-Pepsin.
Host
Sheep
Isotype
IgG
Description
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep IgG, anti-Sheep IgG F(ab')2 and anti-Sheep Serum
Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Applications for Sheep IgG F(ab)2 Isotype Control
Application
Recommended Usage
ELISA
1:100 - 1:2000
Immunohistochemistry
1:10 - 1:500
Western Blot
1:100 - 1:2000
Application Notes
This product has been tested in SDS-Page and are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
Purified
Preservative
0.01% Sodium Azide
Concentration
Please contact technical services for concentration.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Background: IgG F(ab)2
Additional IgG F(ab)2 Products
Product Documents for Sheep IgG F(ab)2 Isotype Control
Product Specific Notices for Sheep IgG F(ab)2 Isotype Control
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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