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Sheep IgG F(ab)2 Isotype Control [HRP]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97124

Novus Biologicals, part of Bio-Techne
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NBP1-97124

Key Product Details

Species Reactivity

Sheep

Applications

ELISA, Immunohistochemistry, Western Blot

Label

HRP

Antibody Source

Sheep IgG

Concentration

LYOPH mg/ml

Product Specifications

Specificity

No reaction was observed against anti-Sheep IgG F(c) or anti-Pepsin.

Host

Sheep

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep IgG, anti-Sheep IgG F(ab')2 and anti-Sheep Serum

Applications for Sheep IgG F(ab)2 Isotype Control [HRP]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This product reagents are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.

NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 1.0 ml deionized water (or equivalent).

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Gentamicin Sulfate

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Background: IgG F(ab)2

Additional IgG F(ab)2 Products

Product Documents for Sheep IgG F(ab)2 Isotype Control [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Sheep IgG F(ab)2 Isotype Control [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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