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Mouse IgG Fc Isotype Control [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97278

Novus Biologicals, part of Bio-Techne
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NBP1-97278

Key Product Details

Species Reactivity

Mouse

Applications

Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Mouse IgG

Concentration

LYOPH mg/ml

Product Summary for Mouse IgG Fc Isotype Control [FITC]

Specificity

No reaction was observed against anti-Mouse IgG F(ab')2 or anti-Papain.

Host

Mouse

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Mouse IgG, anti-Mouse IgG F(c) and anti-Mouse Serum

Scientific Data Images for Mouse IgG Fc Isotype Control [FITC]

Western Blot: Mouse IgG Fc Isotype Control [FITC] [NBP1-97278]

Western Blot: Mouse IgG Fc Isotype Control [FITC] [NBP1-97278]

Western Blot: Mouse IgG Fc Isotype Control [Fluorescein] [NBP1-97278] - Lane 1: 5 uL Opal Prestained Marker. Lane 2: Reduced Mouse IgG Whole Molecule. Lane 3: Reduced Mouse F(c) Fragment Fluorescein Conjugated (NBP1-97278). Lane 4: Reduced Mouse F(ab) Fragment. Lane 5: Mouse IgM Kappa Myeloma Protein. Load: 1 ug per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.

Applications for Mouse IgG Fc Isotype Control [FITC]

Application
Recommended Usage

Fluorophore-linked immunosorbent assay

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 1.0 ml deionized water (or equivalent).

Formulation

Lyophilized from 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Product Documents for Mouse IgG Fc Isotype Control [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse IgG Fc Isotype Control [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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