Mouse IgG Fc Isotype Control [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97293
Forumulation
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Dot Blot, ELISA, SDS-Page, Western Blot
Label
Biotin
Antibody Source
Mouse IgG
Concentration
LYOPH mg/ml
Product Summary for Mouse IgG Fc Isotype Control [Biotin]
Specificity
No reaction was observed against anti-Mouse IgG F(ab')2 or anti-Papain.
Host
Mouse
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Mouse IgG, anti-Mouse IgG F(c) and anti-Mouse Serum
This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Mouse IgG, anti-Mouse IgG F(c) and anti-Mouse Serum
Scientific Data Images for Mouse IgG Fc Isotype Control [Biotin]
Dot Blot: Mouse IgG Fc Isotype Control [Biotin] [NBP1-97293]
SDS-PAGE: Mouse IgG Fc Isotype Control [Biotin] [NBP1-97293]
SDS-Page: Mouse IgG Fc Isotype Control [Biotin] [NBP1-97293] - SDS-PAGE of Mouse IgG Fc Isotype control [Biotin]. Lane 1: 5 uL Opal Prestained Marker. Lane 2: Reduced Mouse IgG Whole Molecule. Lane 3: Reduced Mouse F(c) Fragment Biotin Conjugated. Lane 4: Reduced Mouse F(ab) Fragment. Lane 5: Mouse IgM Kappa Myeloma Protein. Load: 1 ug per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.Applications for Mouse IgG Fc Isotype Control [Biotin]
Application
Recommended Usage
Dot Blot
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
SDS-Page
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This product has been tested in dot blot and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 1.0 ml deionized water (or equivalent).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgG Fc
Additional IgG Fc Products
Product Documents for Mouse IgG Fc Isotype Control [Biotin]
Product Specific Notices for Mouse IgG Fc Isotype Control [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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