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Mouse IgG Fc Isotype Control [HRP]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97299

Novus Biologicals, part of Bio-Techne
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NBP1-97299

Key Product Details

Species Reactivity

Mouse

Applications

SDS-Page

Label

HRP

Antibody Source

Mouse IgG

Concentration

LYOPH mg/ml

Product Specifications

Specificity

No reaction was observed against anti-Mouse IgG F(ab')2 or anti-Papain.

Host

Mouse

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Mouse IgG, anti-Mouse IgG F(c) and anti-Mouse Serum

Scientific Data Images for Mouse IgG Fc Isotype Control [HRP]

SDS-PAGE: Mouse IgG Fc Isotype Control [HRP] [NBP1-97299]

SDS-PAGE: Mouse IgG Fc Isotype Control [HRP] [NBP1-97299]

SDS-Page: Mouse IgG Fc Isotype Control [HRP] [NBP1-97299] - Lane 1: 5 uL Opal Prestained Marker. Lane 2: Reduced Mouse IgG Whole Molecule. Lane 3: Reduced Mouse F(c) Fragment Peroxidase Conjugated. Lane 4: Reduced Mouse F(ab) Fragment. Lane 5: Mouse IgM Kappa Myeloma Protein. Load: 1 ug per lane.

Applications for Mouse IgG Fc Isotype Control [HRP]

Application
Recommended Usage

SDS-Page

1:100 - 1:2000
Application Notes
This product can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 1.0 ml deionized water (or equivalent).

Formulation

Lyophilized from 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Gentamicin Sulfate

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Product Documents for Mouse IgG Fc Isotype Control [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse IgG Fc Isotype Control [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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