Mouse IgG2a Isotype Control (M2A)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-96778
![Immunoprecipitation: Mouse IgG2a Isotype Control (M2A) [NBP1-96778]](https://resources.bio-techne.com/images/products/Mouse-IgG2a-Isotype-Control-M2A-Immunoprecipitation-NBP1-96778-img0001.jpg "Immunoprecipitation: Mouse IgG2a Isotype Control (M2A) [NBP1-96778]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Mouse
Applications
ELISA, Flow Cytometry, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # M2A
Format
Purified
Concentration
Please contact technical services for concentration.
Product Specifications
Specificity
Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgM, and IgA. Confirmed by SDS-PAGE.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Description
This antibody has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2a and anti-Mouse Serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgM, and IgA. Confirmed by SDS-PAGE.
Store vial at 4C prior to opening. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to opening. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Mouse IgG2a Isotype Control (M2A)
Immunoprecipitation: Mouse IgG2a Isotype Control (M2A) [NBP1-96778]
Immunoprecipitation: Mouse IgG2a Isotype Control (M2A) [NBP1-96778] - Cell lysates from T24 cells treated for 3h with vehicle or perifosine (0.5, 1 or 2.5 uM) were immunoprecipitated (IP) using a monoclonal anti-CB Ab. The immunoprecipitates were separated via 12% SDS-PAGE and then immunoblotted (IB) using Abs against phospho-tyrosine (pTyr) and CB. The lysates were also immunoprecipitated using isotype-matched IgG, which was used as a control. Image collected and cropped by CiteAb from the following publication (impactjournals.com/oncoscience/index.php?abs=147), licensed under a CC-BY license.![Immunoprecipitation: Mouse IgG2a Isotype Control (M2A) [NBP1-96778]](https://resources.bio-techne.com/images/products/Mouse-IgG2a-Isotype-Control-M2A-Immunoprecipitation-NBP1-96778-img0002.jpg "Immunoprecipitation: Mouse IgG2a Isotype Control (M2A) [NBP1-96778]")
Immunoprecipitation: Mouse IgG2a Isotype Control (M2A) [NBP1-96778]
Immunoprecipitation: Mouse IgG2a Isotype Control (M2A) [NBP1-96778] - Cell lysates from T24 BC cells either untreated or treated with sorafenib (20 uM) for 1 or 3 h were immunoprecipitated (IP) using a monoclonal anti-CB Ab. The immunoprecipitates were separated via 12% SDS-PAGE and then immunoblotted (IB) using Abs against phospho-tyrosine (anti-pTyr) and CB. The lysates were also immunoprecipitated using isotype-matched IgG, which was used as a control. Image collected and cropped by CiteAb from the following publication (impactjournals.com/oncoscience/index.php?abs=147), licensed under a CC-BY license.
Mouse IgG2a Isotype Control (M2A)
SDS-Page Results of Mouse IgG2a Isotype Control (M2A).Lane 1: Mouse IgG2a Isotype Control (M2A) Reduced [1.0ug]. Lane 2: Opal Prestained Molecular Weight MarkerApplications for Mouse IgG2a Isotype Control (M2A)
Application
Recommended Usage
ELISA
1:100 - 1:2000
Flow Cytometry
1:1000 - 1:5000
Fluorophore-linked immunosorbent assay
1:100 - 1:2000
Immunocytochemistry/ Immunofluorescence
1:10 - 1:500
Western Blot
1:100 - 1:2000
Application Notes
This product has been tested by SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
Purified
Preservative
0.01% Sodium Azide
Concentration
Please contact technical services for concentration.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgG2A
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G2A Fc
Additional IgG2A Products
Product Documents for Mouse IgG2a Isotype Control (M2A)
Product Specific Notices for Mouse IgG2a Isotype Control (M2A)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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