Mouse IgG2a Isotype Control (M2A) [FITC]

Mouse

Dot Blot, ELISA, Flow Cytometry, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Western Blot

FITC (Excitation = 495 nm, Emission = 519 nm)

Monoclonal Mouse IgG_2A Clone # M2A

LYOPH mg/ml

Extensive cross-adsorbtion was performed to remove any unwanted subclasses. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgM, and IgA. Fluorescein Conjugated Mouse IgG2a was proven by Dot Blot.

Monoclonal

Mouse

IgG_2A

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This antibody has been prepared from normal mouse serum by immunoaffinity chromatography using antibodies coupled to agarose beads. Extensive cross-adsorption was performed to remove any unwanted subclasses. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2a and anti-Mouse Serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgM, and IgA. Fluorescein Conjugated Mouse IgG2a was proven by Dot Blot.

Immunogen affinity purified

Reconstitute with 100 ul deionized water (or equivalent).

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.01% Sodium Azide

LYOPH mg/ml

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.