Rat IgM Isotype Control
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-96776
![SDS-PAGE: Rat IgM Isotype Control [NBP1-96776]](https://resources.bio-techne.com/images/products/Rat-IgM-Isotype-Control-SDS-Page-NBP1-96776-img0001.jpg "SDS-PAGE: Rat IgM Isotype Control [NBP1-96776]")
Key Product Details
Species Reactivity
Rat
Applications
ELISA, Immunohistochemistry, SDS-Page, Western Blot
Label
Unconjugated
Antibody Source
Rat IgM
Format
Purified
Concentration
Please contact technical services for concentration.
Product Summary for Rat IgM Isotype Control
Specificity
No reaction was observed against anti-Rat IgG F(c). Some light chain cross-reactivity will occur with anti-Rat IgG.
Host
Rat
Isotype
IgM
Description
This product was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat Serum and anti-Rat IgM (u chain specific)
Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Scientific Data Images for Rat IgM Isotype Control
SDS-PAGE: Rat IgM Isotype Control [NBP1-96776]
SDS-Page: Rat IgM Isotype Control [NBP1-96776] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule. Lane 2: Reduced Rat IgG F(c) Fragment. Lane 3: Reduced Rat IgG Fab Fragment. Lane 4: Reduced Rat IgM Whole Molecule. Load: 1 ug of IgG, F(c), Fab; 1.5 ug of IgM.![SDS-PAGE: Rat IgM Isotype Control [NBP1-96776]](https://resources.bio-techne.com/images/products/Rat-IgM-Isotype-Control-SDS-Page-NBP1-96776-img0002.jpg "SDS-PAGE: Rat IgM Isotype Control [NBP1-96776]")
SDS-PAGE: Rat IgM Isotype Control [NBP1-96776]
SDS-Page: Rat IgM Isotype Control [NBP1-96776] - Lane 1: Rat IgM, Non-Reduced. Lane 2: Rat IgM, Reduced. Load: 1.0 ug per lane.Applications for Rat IgM Isotype Control
Application
Recommended Usage
Immunohistochemistry
1:10 - 1:500
SDS-Page
1:100 - 1:2000
Western Blot
1:100-1:2000
Application Notes
This product has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Format
Purified
Preservative
0.01% Sodium Azide
Concentration
Please contact technical services for concentration.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Background: IgM
Long Name
Immunoglobulin M
Alternate Names
AGM1, DKFZp686I15196, DKFZp686I15212, FLJ00385, immunoglobulin heavy constant mu, MGC104996, MGC52291, MU, VH
Gene Symbol
IGHM
Additional IgM Products
Product Documents for Rat IgM Isotype Control
Product Specific Notices for Rat IgM Isotype Control
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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