Rat IgM Isotype Control [FITC]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97202
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Rat
Applications
SDS-Page
Label
FITC (Excitation = 495 nm, Emission = 519 nm)
Antibody Source
Rat IgM
Concentration
LYOPH mg/ml
Product Specifications
Specificity
No reaction was observed against anti-Rat IgG F(c). Some light chain cross reactivity will occur with anti-Rat IgG.
Host
Rat
Isotype
IgM
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This product was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rat Serum and anti-Rat IgM (u chain specific)
This product was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rat Serum and anti-Rat IgM (u chain specific)
Scientific Data Images for Rat IgM Isotype Control [FITC]
SDS-PAGE: Rat IgM Isotype Control [FITC] [NBP1-97202]
SDS-Page: Rat IgM Isotype Control [Fluorescein] [NBP1-97202] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule. Lane 2: Reduced Rat IgG F(c) Fragment. Lane 3: Reduced Rat IgG Fab Fragment. Lane 4: Reduced Rat IgM Whole Molecule Fluorescein Conjugated. Load: 1 ug of IgG, F(c), Fab; 1.5 ug of IgM.Applications for Rat IgM Isotype Control [FITC]
Application
Recommended Usage
SDS-Page
1:100 - 1:2000
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 500 ul deionized water (or equivalent).
Formulation
Lyophilized from 0.06 M Potassium Phosphate, 0.45 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgM
Long Name
Immunoglobulin M
Alternate Names
AGM1, DKFZp686I15196, DKFZp686I15212, FLJ00385, immunoglobulin heavy constant mu, MGC104996, MGC52291, MU, VH
Gene Symbol
IGHM
Additional IgM Products
Product Documents for Rat IgM Isotype Control [FITC]
Product Specific Notices for Rat IgM Isotype Control [FITC]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...