Mouse IgM Isotype Control
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97007
![SDS-PAGE: Mouse IgM Isotype Control [NBP1-97007]](https://resources.bio-techne.com/images/products/Mouse-IgM-Isotype-Control-SDS-Page-NBP1-97007-img0001.jpg "SDS-PAGE: Mouse IgM Isotype Control [NBP1-97007]")
Key Product Details
Species Reactivity
Mouse
Applications
ELISA, SDS-Page, Western Blot
Label
Unconjugated
Antibody Source
Mouse IgM
Format
Purified
Concentration
Please contact technical services for concentration.
Product Specifications
Specificity
No reaction was observed against anti-Mouse IgG F(c). Some light chain cross-reactivity will occur with anti-Mouse IgG.
Host
Mouse
Isotype
IgM
Description
This product was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum and anti-Mouse IgM (u chain specific)
Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Scientific Data Images for Mouse IgM Isotype Control
SDS-PAGE: Mouse IgM Isotype Control [NBP1-97007]
SDS-Page: Mouse IgM Isotype Control [NBP1-97007] - Lane 1: 5 uL Opal Prestained Marker. Lane 2: Reduced Mouse IgG Whole Molecule. Lane 3: Reduced Mouse F(c) Fragment. Lane 4: Reduced Mouse F(ab) Fragment. Lane 5: Mouse IgM Whole Molecule. Load: 1 ug per lane.![SDS-PAGE: Mouse IgM Isotype Control [NBP1-97007]](https://resources.bio-techne.com/images/products/Mouse-IgM-Isotype-Control-SDS-Page-NBP1-97007-img0002.jpg "SDS-PAGE: Mouse IgM Isotype Control [NBP1-97007]")
SDS-PAGE: Mouse IgM Isotype Control [NBP1-97007]
SDS-Page: Mouse IgM Isotype Control [NBP1-97007] - Lane 1: Mouse IgM, Non-Reduced. Lane 2: Mouse IgM, Reduced. Load: 1.0 ug per lane.Applications for Mouse IgM Isotype Control
Application
Recommended Usage
ELISA
1:100 - 1:2000
SDS-Page
1:100 - 1:2000
Western Blot
1:100 - 1:2000
Application Notes
This product has been tested by SDS-Page and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Format
Purified
Preservative
0.1% Sodium Azide
Concentration
Please contact technical services for concentration.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Background: IgM
Long Name
Immunoglobulin M
Alternate Names
AGM1, DKFZp686I15196, DKFZp686I15212, FLJ00385, immunoglobulin heavy constant mu, MGC104996, MGC52291, MU, VH
Gene Symbol
IGHM
Additional IgM Products
Product Documents for Mouse IgM Isotype Control
Product Specific Notices for Mouse IgM Isotype Control
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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