Mouse IgM Isotype Control [FITC]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97174
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Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
FITC (Excitation = 495 nm, Emission = 519 nm)
Antibody Source
Mouse IgM
Concentration
LYOPH mg/ml
Product Specifications
Specificity
No reaction was observed against anti-Mouse IgG F(c). Some light chain cross reactivity will occur with anti-Mouse IgG.
Host
Mouse
Isotype
IgM
Description
Store vial at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use.
This product was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Mouse Serum and anti-Mouse IgM (u chain specific)
This product was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Mouse Serum and anti-Mouse IgM (u chain specific)
Applications for Mouse IgM Isotype Control [FITC]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 500 ul deionized water (or equivalent).
Formulation
Lyophilized from 0.06 M Potassium Phosphate, 0.45 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: IgM
Long Name
Immunoglobulin M
Alternate Names
AGM1, DKFZp686I15196, DKFZp686I15212, FLJ00385, immunoglobulin heavy constant mu, MGC104996, MGC52291, MU, VH
Gene Symbol
IGHM
Additional IgM Products
Product Documents for Mouse IgM Isotype Control [FITC]
Product Specific Notices for Mouse IgM Isotype Control [FITC]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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