Human IL-15 XL Luminex® Performance Assay

Name: Human IL-15 XL Luminex® Performance Assay
Brand: R&D Systems
SKU: LUXLM247

R&D Systems, part of Bio-Techne | Catalog # LUXLM247

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Luminex High Performance Assays

Luminex High Performance Assays go through rigorous development, validation, and quality control testing to ensure you are getting the best performance out of your multiplex assay. Panels are customizable so you can order a selection of the analytes or the entire panel.

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Key Product Details

Panel:	Luminex Performance Human XL Cytokine Panel
Assay Type:	Magnetic bead-based multiplex assay for the Luminex® MAGPIX® platform
Format:	1 x 96-well microplate and magnetic antibody-coated beads
Species:	Human
Analytes Detected:	IL-15

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View Full Technical Details for Human IL-15 XL Luminex® Performance Assay

Technical Data
Citations / Reviews

Product Features for Human IL-15 XL Luminex® Performance Assay

Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.

Simultaneously profile Human IL-15 with up to 45 analytes of your choice in one sample
Magnetic format allows for easier wash steps
Undergoes similar validation testing as Quantikine® ELISA Kits
Precise and Specific
Requires a small sample volume (<50 µL)
Run your assay in just 3-5 hours
Mass-calibrated standards for consistent results with every new lot of material
Enough reagents for one 96-well plate

Performance Validation for Human IL-15 XL Luminex® Performance Assay

Our Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.

View Luminex High Performance Human XL validation data.

Kit Components for Human IL-15 XL Luminex® Performance Assay

Premixed cocktail of biotinylated detection antibody
Standard Cocktail(s)
Bead Diluent
Biotin Antibody Diluent
Calibrator Diluent
Wash Buffer
Streptavidin-PE
One flat-bottom 96-well Microplate
Foil Plate Sealers (4)
Mixing Bottle
Standard Value Card**
High and low controls with Control Mean Value Card

**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.

Bead Set Contents

User-mix antibody-coated Magnetic beads in individual vials

Recommended Sample Types: Cell culture supernates, serum, EDTA plasma, and heparin plasma

Components: Human IL-15 Magnetic Microparticles (Part 898838) is supplied as a 100X concentrated stock (0.075 mL) with preservatives. Other Supplies Required: Magnetic Luminex® Performance Assay Human XL Discovery Base Kit (R&D Systems®, Catalog # LUXLM000).

Sensitivity: The Minimum Detectable Dose (MDD) was determined by adding two standard deviations to the mean MFI of twenty zero standard replicates and calculating the corresponding concentration. Six assays were evaluated, and the MDD of human IL-15 ranged from 0.104-0.900 pg/mL. The mean MDD was 0.257 pg/mL.

Correlation: This assay has been correlated to the Quantikine® ELISA Kit.

Specificity: This assay recognizes natural and recombinant human IL-15.

Bead Region: 47

Precision

	Intra-Assay Precision		Inter-Assay Precision
Intra-assay Precision (precision within an assay) - Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (precision between assays) - Two samples of known concentration were tested in thirty separate assays to assess precision between assays.
Sample	1	2	1	2
n	20	20	30	30
Mean (pg/mL)	24.5	239	25.0	248
Standard deviation	0.74	7.93	3.67	19.4
CV (%)	2.0	31.7	14.6	81.2

Recovery

Sample Type	Average % Recovery	Range (%)
Samples containing and/or spiked with human IL-15 were evaluated for recovery.
Cell culture supernate	102	95.4-115
Serum	75.8	66.6-90.8
EDTA plasma	80.9	73.1-87.5
Heparin plasma	79.2	73.3-92.3
Plaelet-poor EDTA	87.1	63.9-111
Platelet-poor Heparin	86.7	67.7-110

Linearity

		Cell culture supernate	Serum	EDTA plasma	Heparin plasma	Platelet-poor EDTA	Platelet-poor Heparin
Samples containing and/or spiked with human IL-15 were serially diluted to evaluate assay linearity.
1:2	Average % of Expected	99.5	112	107	112	110	110
1:2	Range (%)	98.2-101	108-120	103-112	108-119	100-118	102-116
1:4	Average % of Expected	95.8	102	97.5	100	107	110
1:4	Range (%)	93.3-98.5	95.8-107	92.5-108	95.1-105	101-114	101-129
1:8	Average % of Expected	98.7	100	95.0	97.1	106	99.5
1:8	Range (%)	94.9-102	98.6-105	88.6-105	90.2-105	96.5-124	90.8-103
1:16	Average % of Expected	99.1	96.6	89.3	90.4	99.9	92.0
1:16	Range (%)	96.6-102	90.1-103	83.5-101	86.3-99.0	95.4-107	82.9-97.4

Build Your Assay

Make Your Selections Below to Configure Your Custom Luminex Assay

Assays

Below are kits that are available based on your selections. These selections may be further customized before adding to the cart or emailing the configuration to your purchasing department or local distributor to place your order. Selected analytes may be included in more than one High Performance Assay. All assay options are displayed.

If you run into bead region incompatibilities, send your Luminex code to our team and find out more.

How would you like these analytes mixed?

Premixed

User Mixed

Please select at least one mixing option to see the kits.

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Do not split

Split based on serum

Split based on plasma

Split based on cell culture supernates

Which Luminex Assay is Right For Me?

Assays for the Luminex platform are offered as High Performance Assays or Discovery Assays . The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available as Configurable User Mix and Premixed options or as Fixed Panels. The Luminex Discovery Assays allow for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays on rndsystems.com .

More Information About Magnetic Bead-based Multiplex Kits For The Luminex® Platform

Magnetic Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 200™ or FLEXMAP 3D, dual laser, flow-based sorting and detection platforms.

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using a Luminex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaMagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a FLEXMAP 3D dual laser, flow-based systems.

Scientific Data Examples for Human IL-15 XL Luminex® Performance Assay

	IL-15 XL Cytokine Luminex Panel Standard Curves

Background: IL-15

IL-15 (Interleukin 15) is a cytokine that induces or enhances the differentiation, maintenance, or activation of multiple T cell subsets including NK, NKT, Th17, Treg, and CD8+ memory cells. It also induces dendritic cell differentiation and inflammatory activation, exhibits anti-tumor activity, and inhibits the deposition of lipid in adipocytes. Complexes of IL-15 with cell surface IL-15 R alpha interact with complexes of IL-2 R beta and the Common gamma Chain on adjacent cells. This transpresentation mechanism enables cells to respond to IL-15 even if they do not express IL-15 R alpha. Ligation of membrane-associated IL-15/IL-15 R alpha complexes also induces reverse signaling that promotes activation of the IL-15/IL-15 R alpha expressing cells. The activity of circulating IL-15 is limited by its association with soluble IL-15 R alpha.

Item	Value
Long Name	Interleukin 15
Entrez Gene IDs	3600 (Human); 16168 (Mouse); 25670 (Rat); 102119613 (Cynomolgus Monkey); 493682 (Feline)
Alternate Names	IL-15, IL-15MGC9721, IL15, interleukin 15, interleukin-15

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Product Specific Notices for Human IL-15 XL Luminex® Performance Assay

Luminex® is a registered trademark of Luminex Corporation.

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