I just received APC Partial Recombinant Protein (H00000324-Q01) (10 ug), which I want to use as a positive control in a WB. What is the recommended amount of this protein to load?

For WB application with APC Partial Recombinant Protein (H00000324-Q01), you may load 0.1-0.2 ug of the protein per lane.

I want to use APC Partial Recombinant Protein (H00000324-Q01) as a positive control in a WB. What is your recommendation for the amount of this protein to load in a 10-well gel?

H00000324-Q01 is a product we distribute for a Taiwanese company called Abnova. They suggest loading their recombinant proteins at 0.1-0.2ug per lane.

Recombinant Human APC GST (N-Term) Protein

Name: Recombinant Human APC GST (N-Term) Protein
Brand: Novus Biologicals
SKU: H00000324-Q01

Novus Biologicals, part of Bio-Techne | Catalog # H00000324-Q01

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Product Datasheet / COA / SDS

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

Wheat germ

Tag

GST (N-Term)

Conjugate

Unconjugated

Applications

ELISA, Affinity Purification, Microarray, Western Blot

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Product Specifications

Description

A recombinant protein with a N-terminal GST tag corresponding to the amino acid sequence 2744-2843 of Human APC

Source: Wheat Germ (in vitro)

Amino Acid Sequence: NPVPVSETNESSIVERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVNNNTKKRDSKTDSTESSGTQSPKRHSGSYLVTSV

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

36.85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human APC GST (N-Term) Protein

SDS-PAGE: Recombinant Human APC GST (N-Term) Protein [H00000324-Q01]

SDS-Page: Recombinant Human APC Protein [H00000324-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

Formulation, Preparation and Storage

H00000324-Q01

Preparation Method	in vitro wheat germ expression system
Formulation	50 mM Tris-HCI, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Preservative	No Preservative
Concentration	Please see the vial label for concentration. If unlisted please contact technical services.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -80C. Avoid freeze-thaw cycles.

Background: APC

APC - adenomatosis polyposis coli

Long Name

Adenomatosis Polyposis Coli

Alternate Names

DP2, DP3, FPC, GS, Min

Gene Symbol

APC

Additional APC Products

Product Documents for Recombinant Human APC GST (N-Term) Protein

Product Datasheets

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COA

SDS

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Product Specific Notices for Recombinant Human APC GST (N-Term) Protein

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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