Biotinylated Recombinant Human LAIR2 Fc Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI10166

Biotinylated
R&D Systems, part of Bio-Techne
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

NP_002279.2

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all LAIR2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human LAIR2 protein
Human LAIR2
(Gln22-Pro152)
Accession # NP_002279.2		 IEGRMD Human IgG1
(Pro100-Lys333)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln22; deduced from Glu23 upon deblocking

Predicted Molecular Mass

42 kDa

SDS-PAGE

50-59 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Bovine Collagen I is coated at 10 µg/mL (100 μL/well), Biotinylated Recombinant Human LAIR2 Fc Chimera Avi-tag (Catalog # AVI10166) binds with an ED50 of 0.175-1.4 ng/mL.

Scientific Data Images for Biotinylated Recombinant Human LAIR2 Fc Avi-tag Protein, CF

Biotinylated Recombinant Human LAIR2 Fc Chimera Avi-tag Binding Activity.

When Bovine Collagen I is coated at 10 µg/mL (100 μL/well), Biotinylated Recombinant Human LAIR2 Fc Chimera Avi-tag (Catalog # AVI10166) binds with an ED50 of 0.175-1.4 ng/mL.

Biotinylated Recombinant Human LAIR2 Fc Chimera Avi-tag SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human LAIR2 Fc Chimera Avi-tag Protein (AVI10166) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-59 kDa and 100-120 kDa, respectively.

Formulation, Preparation and Storage

AVI10166
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAIR2

LAIR2 (leukocyte-associated Ig-like receptor-2; CD306) is a secreted, 131 amino acid (aa) protein that contains one Ig-like C2 type domain, making it a member of the Ig superfamily. When compared to LAIR1, its transmembrane counterpart, LAIR2 shares 83% aa identity across the signal sequence and extracellular domains (1-3). Although one is secreted and the other is membrane-bound, the two LAIR proteins are thought to have arisen from a common gene ancestor and appear to share similar adhesion profiles. This suggests that LAIR2 may compete with LAIR1 for ligand binding (3, 4). A 114 aa alternate splice form of LAIR-2 is truncated at the C-terminus, but retains the entire Ig domain (1-3). The expression profile of these splice forms, and the presence of orthologs in other species, have not been reported. LAIR2 is a soluble collagen-receptor, and it can be detected in the synovial fluid of rheumatoid arthritis patients, urine of pregnant women, and as well as primary cells (5, 6). In vitro studies have demonstrated LAIR2 can compete with LAIR1 for the same collagen binding site and suggesting LAIR2 may play an important role in immune cell activation (5, 6). LAIR2 can interact with complement component 1q (C1q) and mannose-binding lectin (MBL) and act as a complement inhibitor for the treatment and prevention of antibody-mediated allograft rejection and antibody-mediated clinical conditions (7). Our Avi-tag Biotinylated human LAIR2 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Meyaard, L. (2003) J. Biol. Regul. Homeost. Agents 17:330.
  2. Meyaard, L. et al. (1999) J. Immunol. 162:5800.
  3. Meyaard, L. et al. (1997) Immunity 7:283.
  4. Xu, X.G. et al. (2005) Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 21:553.
  5. Lebbink, R.J. et al. (2008) J. Immunol. 180:1662.
  6. Olde Nordkamp, M.J. et al. (2011) Arthritis Rheum. 63:3749.
  7. Olde Nordkamp, M.J. et al. (2014) J. Innate Immun. 6:284.

Long Name

Leukocyte-associated Ig-like Receptor 2

Alternate Names

CD306

Entrez Gene IDs

3904 (Human); 102141809 (Cynomolgus Monkey)

Gene Symbol

LAIR2

UniProt

NP_002279.2

Additional LAIR2 Products

Product Documents for Biotinylated Recombinant Human LAIR2 Fc Avi-tag Protein, CF

Product Datasheets

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Product Specific Notices for Biotinylated Recombinant Human LAIR2 Fc Avi-tag Protein, CF

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