Recombinant Rat FGF-21 Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35194
Key Product Details
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 180 amino acids corresponding to FGF-21 Source: E. coli
Uniprot ID: Q8VI80
Amino Acid Sequence: AYPISDSSPL LQFGGQVRQR YLYTDDDQDT EAHLEIREDG TVVGTAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD GTLYGSPHFD PEACSFRELL LKDGYNVYQS EAHGLPLRLP QKDSQDPATR GPVRFLPMPG LPHEPQEQPG VLPPEPPDVG SSDPLSMVEP LQGRSPSYAS
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 1 EU/ug of FGF-21 as determined by LAL method.
Predicted Molecular Mass
19.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
FGF-21 protein is fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ug/ml, corresponding to a specific activity of > 2.0 x 10^3 IU/mg in the presence of 5 ug/ml of rMuKlotho-beta and 10 ug/ml of heparin.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Rat FGF-21 Protein
![SDS-PAGE: Recombinant Rat FGF-21 Protein [NBP2-35194]](https://resources.bio-techne.com/images/products/Rat-FGF-21-Protein-SDS-Page-NBP2-35194-img0001.jpg "SDS-PAGE: Recombinant Rat FGF-21 Protein [NBP2-35194]")
SDS-PAGE: Recombinant Rat FGF-21 Protein [NBP2-35194]
SDS-Page: Rat FGF-21 Protein [NBP2-35194]Formulation, Preparation and Storage
NBP2-35194
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in PBS, 500 mM NaCl, pH 7.4. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: FGF-21
Additional FGF-21 Products
Product Documents for Recombinant Rat FGF-21 Protein
Product Specific Notices for Recombinant Rat FGF-21 Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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