hi I was wondering if the recombinant human galectin-1 protein (NBP2-34878-100ug) has a tag on it?

Name: Recombinant Human Galectin-1 Protein
Brand: Novus Biologicals
SKU: NBP2-34878

Recombinant Human Galectin-1 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34878

Submit a review

Citations (1)

Product Datasheet / COA

Catalog #

Availability

Size / Price

Qty

100% Guarantee

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P09382

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all Galectin-1 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 134 amino acids corresponding to Galectin-1 Source: E. coli

Uniprot ID: P09382

Amino Acid Sequence: ACGLVASNLN LKPGECLRVR GEVAPDAKSF VLNLGKDSNN LCLHFNPRFN AHGDANTIVC NSKDGGAWGT EQREAVFPFQ PGSVAEVCIT FDQANLTVKL PDGYEFKFPN RLNLEAINYM AADGDFKIKC VAFD

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of Galectin-1 as determined by LAL method.

Predicted Molecular Mass

14.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Galectin 1 protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human blood monocytes is in a concentration range of 1.0-10 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Galectin-1 Protein

SDS-PAGE: Recombinant Human Galectin-1 Protein [NBP2-34878]

SDS-Page: Human Galectin-1 Protein [NBP2-34878]

Formulation, Preparation and Storage

NBP2-34878

Formulation	Lyophilized from a 0.2 um filtered solution in PBS pH 7.0, 3mM beta-Me, 0.02% Tween 20.
Preservative	5% Trehalose
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Galectin-1

Human Galectin-1 also named BHL, Galaptin, GBP, L-14 and LGALS1, is belonging to the galectins family and it is encoded by the LGALS1 gene in human. Galectin-1 is expressed by the endometrial stromal cells throughout the menstrual cycle. Galectin-1 contains a single carbohydrate recognition domain through which it can bind glycans both as a monomer and as a homodimer. Dimers are non-covenantly bound and will spontaneously disassociate in low concentration. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Galectin-1 is thought to play a role in the immunosuppression required for a successful pregnancy. Human Galectin-1 shares 88 %-90 % amino acid sequence identity with rat and mouse.

Alternate Names

BHL, Galaptin, Galectin1, GBP, L-14, LGALS1

Gene Symbol

LGALS1

UniProt