Recombinant Rat GDNF Protein

Name: Recombinant Rat GDNF Protein
Brand: Novus Biologicals
SKU: NBP2-35204

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35204

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Product Datasheet / COA

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Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

Q07731

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all GDNF Proteins and Enzymes »

Product Specifications

Description

A homodimeric protein consisting of two 134 amino acid non-glycosylated polypeptide chains corresponding to GDNF Source: E. coli

Uniprot ID: Q07731

Amino Acid Sequence: SPDKQAAALP RRERNRQAAA ASPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCEAAETMYD KILKNLSRSR RLTSDKVGQA CCRPVAFDDD LSFLDDSLVY HILRKHSAKR CGCI

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of GDNF as determined by LAL method.

Predicted Molecular Mass

29.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

GDNF protein is fully biologically active when compared to standard. Measured by its ability to support the survival and stimulate neurite outgrowth of dissociated chick embryonic dorsal root ganglia (DRG) neurons. The ED50 for this effect is typically 1 - 3 ng/mL

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-35204

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 1 x PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: GDNF

Glial cell-derived neurotrophic factor is a founding member of the GDNF family of ligands (GFL) and has been shown to interact with GFRA2 and GDNF family receptor alpha 1. It is a small protein that potently promotes the survival and morphological differentiation of various neuronal. It may also modulate local neuronal effects in distal regions of the motor neuron. GDNF Recombinant rat GDNF (monomer) contains 135 amino acids residues, which is a disulfide-linked homodimer and it shares 99 % and 93 % a.a. sequence identity with mouse and human GDNF.

Long Name

Glial Cell line-derived Growth Factor

Alternate Names

ATF, HFB1-GDNF, HGDNF, HSCR3

Gene Symbol

GDNF

UniProt