I would like to know the isotype of the HMGB1 peptide (NB100-23222PEP), the species of origin, how it was produced, and whether it can be used for immunostimulatory assays or just blocking?

Name: HMGB1/HMG-1 Antibody Blocking Peptide
Brand: Novus Biologicals
SKU: NB100-2322PEP

NB100-2322PEP is a blocking peptide specific to our HMGB1 antibody, NB100-2322. This product has only been tested as a blocking peptide for that specific antibody, and we cannot guarantee any other performance. This peptide corresponds to a internal portion of the human protein and since this is the antigen, there is no specified isotype. The production method of this peptide is proprietary, and unfortunately, I do not have that information available on my end.

HMGB1/HMG-1 Antibody Blocking Peptide

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2322PEP

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Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Conjugate

Unconjugated

Applications

Antibody Competition

View all HMGB1/HMG-1 Proteins and Enzymes »

Product Specifications

Description

A HMGB1 antibody blocking peptide.

Application Notes

This peptide is useful as a blocking peptide for NB100-2322. For further blocking peptide related protocol, click here.

Specificity

This peptide is specific for NB100-2322 only.

Protein / Peptide Type

Antibody Blocking Peptide

Formulation, Preparation and Storage

NB100-2322PEP

Formulation	Peptide dissolved in dH2O. Contains no BSA.
Preservative	No Preservative
Concentration	1.0 mg/ml
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -80C. Avoid freeze-thaw cycles.

Background: HMGB1/HMG-1

HMGB1 and HMGB2 are part of the chromatin non-histone high mobility group proteins 1 and 2. HMGB1 and HMGB2 (containing multiple HMG-boxes) are conserved domains of 80 amino acids which mediate the DNA binding of many proteins. HMG box domains recognize DNA structure. Both HMGB1 and HMGB2 contain a N-terminal HMG box, a central HMG box, and an acidic carboxy terminus. The acidic tails of HMGB1 and HMGB2 contain multiple serine residues which match the phosphorylation consensus sites of casein kinase II, and phosphorylation of this domain appears to be important for proper functioning of these proteins. HMGB1 and HMGB2 have been shown to facilitate the binding of various sequence-specific transcription factors to their respective DNA binding sites. HMGB1 and HMGB2 may also serve as architectural factors that recognize and mediate DNA structural changes that accompany various events such as DNA repair, transcription, and replication.

Long Name

High Mobility Group Protein 1

Alternate Names

HMG-1, HMG1

Gene Symbol

HMGB1

Additional HMGB1/HMG-1 Products

Product Documents for HMGB1/HMG-1 Antibody Blocking Peptide

Product Datasheets

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COA

Product Specific Notices for HMGB1/HMG-1 Antibody Blocking Peptide

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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