Recombinant Mouse HMGB1/HMG-1 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-42888

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-42888-0.1mg
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, ELISA

View all HMGB1/HMG-1 Proteins and Enzymes »

Product Specifications

Description

Recombinant protein corresponding to Met1-Glu215 of mouse HMGB-1 with an N-terminal His-tag. Accession #: NM_010439.

Source: E.coli

Purity

>98%, by SDS-PAGE

Endotoxin Level

Less than 0.01 ng/ug cytokine as determined by the LAL assay.

Predicted Molecular Mass

27.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

The ED50 of this protein, as measured by mouse TNFa induction in RAW264.7 cells, is 3-25 ug/ml in the presence of 10 ug/ml Polymyxin B. This corresponds to a specific activity of 3,300-40 Units/mg.

Application Notes

ED50 = 3 - 25 ug/mL as measured by the induction of TNF-alpha expression in RAW264.7 cells in the presence of 10 ug/mL Polymyxin B.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP1-42888
Formulation Sterile liquid; 20 mM Tris-HCl, pH 8.0, 0.2 M NaCl, 1 mM DTT.
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -80C. Avoid freeze-thaw cycles.

Background: HMGB1/HMG-1

High-mobility group box-1 (HMGB1) protein was originally described as a nuclear non-histone DNA binding chromosomal protein. However, recent studies indicate that damaged, necrotic cells liberate HMGB1 into the extracellular milieu where it functions as a proinflammatory cytokine. Mouse HMGB1 is expressed as a 215 amino acid single chain polypeptide containing three domains: two tandem-linked positively charged DNA-binding domains (HMG box A, aa 9-79 and box B, aa 89-162), and a negatively charged 30 aa C-terminal acidic tail region. Residues 28 - 44 and 180 - 185 contain a nuclear localization signal (NLS). The cytokine activity of HMGB1 is contained in the B box, while the A box is associated with the helix-loop-helix domain of transcription factors. HMGB1 acts both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, as well as a mediator of T cell-dendritic cell interaction. HMGB1 is secreted and acts to transduce cellular signals through its high affinity receptor, RAGE and possibly, TLR2 and TLR4.

Long Name

High Mobility Group Protein 1

Alternate Names

HMG-1, HMG1

Gene Symbol

HMGB1

Additional HMGB1/HMG-1 Products

Product Documents for Recombinant Mouse HMGB1/HMG-1 Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Mouse HMGB1/HMG-1 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.