Recombinant Human IL-10 Animal-Free Protein

Name: Recombinant Human IL-10 Animal-Free Protein
Brand: Novus Biologicals
SKU: NBP2-76208

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76208

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-10 Proteins and Enzymes »

Product Specifications

Description

An un-tagged bioactive recombinant protein corresponding to Human IL-10

Source: E. coli

Amino Acid Sequence: MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N

Produced with no materials of animal or human origin using ANIMAL-FREE purification processes.

Production and Purification

No animal-derived ingredients, No FBS
E. coli based production system
Animal-Free facility, fermentor, columns, filters, labware, lyophilization, and storage

Quality Assurance

Endotoxin levels at <1 EUs/ug
Purity levels at 95% or greater

Purity

>95%, by SDS-PAGE

Endotoxin Level

Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Predicted Molecular Mass

18.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

MC/9 cell Proliferation, >2 x 10^5 units/mg (ED50 acceptance criteria are <5 ng/mL).

Specificity

IL-10 is a non-covalent homodimer, 18.8 kDa (161 aa)

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human IL-10 Animal-Free Protein

SDS-PAGE: Recombinant Human IL-10 Animal-Free Protein [NBP2-76208]

SDS-Page: Recombinant Human IL-10 Animal-Free Protein [NBP2-76208] - 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel.

Bioactivity: Recombinant Human IL-10 Animal-Free Protein [NBP2-76208] - IL-10 induced proliferation of MC9 cells.

Formulation, Preparation and Storage

NBP2-76208

Preparation Method	Protein is derived from genetically modified E. coli.
Formulation	Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Sterile water at 0.1 mg/mL
Format	Animal Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-10

Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Human IL10 is a non glycosylated polypeptide consisting of 178 amino acids. There is 73% homology between the human and mouse IL10 proteins, however, the human IL10 acts on both human and mouse target cells, while the mouse IL10 has species specific activity. The cellular sources of IL10 are CD4+ T cells and T cell clones, thymocytes, B cells and B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL10 will stimulate the growth of stem cells, mast cells and thymocytes. IL10 enhances cytotoxic T cell development, and costimulates B cell differentiation and immunoglobulin secretion. IL10 inhibits cytokine production by macrophages and suppresses macrophage class II MHC expression. The human IL10 gene is on human chromosome 1.

Long Name

Interleukin 10

Alternate Names

CSIF, GVHDS, IL10, IL10A, TGIF

Gene Symbol

IL10

Additional IL-10 Products

Product Documents for Recombinant Human IL-10 Animal-Free Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human IL-10 Animal-Free Protein

Lot Specific Information:
Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.

Detailed Reconstitution:
Centrifuge vial before opening. Suspend the product by gently pipetting the recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots.

Expiration Date:
12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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