Recombinant Equine IL-2 Protein

Name: Recombinant Equine IL-2 Protein
Brand: Novus Biologicals
SKU: NBP2-34950

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34950

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-2 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 129 amino acids corresponding to IL-2 Source: E. coli

Uniprot ID: P37997

Amino Acid Sequence: APTSSSKRET QQQLKQLQMD LKLLLEGVNN NKNPKLSKML TFKINMPKKA TELKHLQCLE EELKPLEEML KNFLSKDIKE LMSNINVTVL GLKGSETRFT CEYDDETGTI VEFLNKWITF SQSIFSTMT

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of IL-2 as determined by LAL method.

Predicted Molecular Mass

14.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 1.0 ug/ml, corresponding to a specific activity of > 1000 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Equine IL-2 Protein

SDS-PAGE: Recombinant Equine IL-2 Protein [NBP2-34950]

SDS-Page: Equine IL-2 Protein [NBP2-34950]

Formulation, Preparation and Storage

NBP2-34950

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 6.5.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-2

IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, gamma delta T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for Tcell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55 kDa IL2R alpha, 75 kDa IL2R beta, 64 kDa common gamma chain IL2R gamma) that are present on the cell surface in varying preformed complexes. Recombinant equine IL-2 is a 14.9 kDa protein containing 129 amino acid residues. At the amino acid sequence level, equine IL-2 shares 72 %, 70 %, 56 % and 54 % sequence similarities with human, porcine, rat and murine IL-2, respectively. It has been reported that equine IL-2 augmented proliferation in equine peripheral blood mononuclear cells, but has no effect on murine CTLL2 cells.

Long Name

Interleukin 2

Alternate Names

Aldesleukin, IL2, Proleukin, TCGF

Gene Symbol

IL2

Additional IL-2 Products

Product Documents for Recombinant Equine IL-2 Protein

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COA

Product Specific Notices for Recombinant Equine IL-2 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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