Recombinant Human IL-2 Protein

Name: Recombinant Human IL-2 Protein
Brand: Novus Biologicals
SKU: NBP2-35054

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35054

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P60568

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-2 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 133 amino acids corresponding to IL-2 Source: E. coli

Uniprot ID: P60568

Amino Acid Sequence: APTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFCQSIIS TLT

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1.0 EU/ug of IL-2 as determined by LAL method.

Predicted Molecular Mass

15.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL2 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 x 10^7 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human IL-2 Protein

SDS-PAGE: Recombinant Human IL-2 Protein [NBP2-35054]

SDS-Page: Human IL-2 Protein [NBP2-35054]

Formulation, Preparation and Storage

NBP2-35054

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 3.5.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. Do not reconstitute in cell culture media directly.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-2

IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, gamma delta T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required forT-cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55 kDa IL-2R alpha, 75 kDa IL-2R beta, and 64 kDa common gamma chain gammac/IL-2Rgamma) that are present on the cell surface in varying preformed complexes. Mature human IL-2 shares 56 % and 66 % amino acid sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity

Long Name

Interleukin 2

Alternate Names

Aldesleukin, IL2, Proleukin, TCGF

Gene Symbol

IL2

UniProt