I purchased the Rat PDGF-BB protein, but I cannot find the specificity. Does it cross-react only with RAT or also with other species?

Name: Recombinant Rat PDGF-BB Protein
Brand: Novus Biologicals
SKU: NBP2-35285

Regarding the specificity of the active protein, the homology of this amino acid sequence is quite high among rats and other rodents~98-100% and with primates there is about ~89% homology. Therefore, one might assume that you could use this active protein in assays for some animals other than rats. I am not sure what the parameters are for your assay are but if you tell me more about it, I might be able to provide more specific information. If you need an antibody to this protein, here are 3 antibodies to the human protein that have not been validated in any other species.

Recombinant Rat PDGF-BB Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35285

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all PDGF-BB Proteins and Enzymes »

Product Specifications

Description

A disulfide-linked homodimeric protein containing two 109 amino acid polypeptide (B chain) chains corresponding to PDGF-BB Source: E. coli

Uniprot ID: Q05028

Amino Acid Sequence: SLGSLAAAEP AVIAECKTRT EVFQISRNLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RASQVQMRPV QVRKIEIVRK KPVFKKATVT LEDHLACKCE TVVTPRPVT

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of PDGF-BB as determined by LAL method.

Predicted Molecular Mass

24.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

PDGF-BB Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 x 10^5 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat PDGF-BB Protein

SDS-PAGE: Recombinant Rat PDGF-BB Protein [NBP2-35285]

SDS-Page: Rat PDGF-BB Protein [NBP2-35285]

Formulation, Preparation and Storage

NBP2-35285

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 1 x PBS, pH 6.9.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: PDGF-BB

Platelet-derived growth factor is a dimeric glycoprotein composed of two A (-AA) or two B (-BB) chains or a combination of the two (-AB). All PDGFs function as secreted, disulphide-linked homodimers, but only PDGFA and B can form functional heterodimers. PDGFs are mitogenic during early developmental stages, driving the proliferation of undifferentiated mesenchyme and some progenitor populations. PDGF is a required element in cellular division for fibroblasts, a type of connective tissue cell that is especially prevalent in wound healing. In essence, the PDGFs allow a cell to skip the G1 checkpoints in order to divide. Recombinant rat PDGF-AA contains 109 amino acids and has a molecular mass of 12.2 kDa.

Long Name

Platelet-derived Growth Factor BB

Alternate Names

PDGFBB

Gene Symbol

PDGFB

Additional PDGF-BB Products

Product Documents for Recombinant Rat PDGF-BB Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Rat PDGF-BB Protein

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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