Recombinant Cotton Rat IL-2 (Cys146Tyr) Protein

R&D Systems, part of Bio-Techne | Catalog # 579-R2

R&D Systems, part of Bio-Techne
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Carrier Free
579-R2-020/CF
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With Carrier
579-R2-020
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Key Product Details

Source

E. coli

Accession #

AAK94012

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-2 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived cotton rat IL-2 protein
Ala21-Gln155 (Cys146Tyr), with and without an N-terminal Met

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met & Ala21

Predicted Molecular Mass

15.6 kDa

Activity

Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295.
The ED50 for this effect is 0.15-0.6 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 579-R2
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 579-R2/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-2

Interleukin 2 (IL-2) is a protein that has a variety of immunological functions, the most notable being the ability of IL-2 to promote the proliferation and maturation of activated T cells. Other biological activities attributed to IL-2 include:

  • Induction of secretion of IFN-gamma and TNF-alpha and -beta from peripheral blood mononuclear cells.
  • Stimulation of the rate of synthesis of c-myc RNA and transferrin receptor.
  • Activation of neutrophils.
  • Stimulation of proliferation and maturation of activated helper T cells.
  • Stimulation of proliferation of activated and natural killer cells and tumor-infiltrating lymphocytes, as well as enhancement of the ability of these cytotoxic lymphocytes to kill target cells.
  • Induction of the expression of IL-2 receptors on T cells stimulation of proliferation of antibody-producing B cells.

    • Long Name

    Interleukin 2

    Alternate Names

    Aldesleukin, IL2, Proleukin, TCGF

    Entrez Gene IDs

    3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)

    Gene Symbol

    IL2

    UniProt

    AAK94012

    Additional IL-2 Products

    Product Documents for Recombinant Cotton Rat IL-2 (Cys146Tyr) Protein

    Product Datasheets

    Certificate of Analysis

    To download a Certificate of Analysis, please enter a lot number in the search box below.

    Note: Certificate of Analysis not available for kit components.

    Download SDS 579-R2 (with carrier)
    Download SDS 579-R2/CF (carrier free)

    Product Specific Notices for Recombinant Cotton Rat IL-2 (Cys146Tyr) Protein

    For research use only

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