Recombinant Cynomolgus Integrin alpha V beta 3 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10426-AV

R&D Systems, part of Bio-Techne
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10426-AV-050
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Key Product Details

Source

CHO

Accession #

XP_005573729.1 (Integrin alphaV) & XP_005584667.1 (Integrin beta3)

Conjugate

Unconjugated

Applications

Bioactivity

View all Integrin alpha V beta 3 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey Integrin alpha V beta 3 protein
Cynomolgus Monkey Integrin alphaV
(Phe31-Val992)
Accession # XP_005573729.1		 His-Pro 2x GGGSGGGS-Acidic Tail  6-His tag
Cynomolgus Monkey Integrin beta3
(Gly27-Asp718)
Accession # XP_005584667.1		 His-Pro 2x GGGSGGGS-Basic tail HA tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe31 (Integrin alphaV) & Gly27 (Integrin beta3)

Predicted Molecular Mass

115 kDa (Integrin alphaV) & 86 kDa (Integrin beta3)

SDS-PAGE

105-160 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Human Vitronectin (Catalog # 2308-VN) is immobilized at 2 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey Integrin alphaV beta3 (Catalog # 10426-AV) binds with an ED50 of 0.06-0.72 μg/mL.

Scientific Data Images for Recombinant Cynomolgus Integrin alpha V beta 3 Protein, CF

Recombinant Cynomolgus Monkey Integrin alpha V beta 3 Protein Binding Activity.

When Recombinant Human Vitronectin (Catalog # 2308-VN) is immobilized at 2 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey Integrin aV beta3 (Catalog # 10426-AV) binds with an ED50 of 0.06-0.72 µg/mL.

Recombinant Cynomolgus Monkey Integrin alpha V beta 3 Protein SDS-PAGE.

2 μg/lane of Recombinant Cynomolgus Monkey Integrin alphaV beta3 Protein (Catalog # 10426-AV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 105-160 kDa.

Formulation, Preparation and Storage

10426-AV
Formulation Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Integrin alpha V beta 3

Integrin alphaV beta3 together with alphaIIb beta3, constitutes the only known beta3 Integrins (1­3). The non­covalent heterodimer of 170 kDa alphaV/CD51 and 93 kDa beta3/CD61 subunits shows wide expression, notably by endothelial cells and osteoclasts (2­4). Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. Active cell surface alphaV beta3 adheres to matrix proteins including vitronectin, fibronectin, fibrinogen and thrombospondin (2, 3). The ligand binding site of alphaV beta3 is in the N­terminal head region, formed by interaction of the beta3 vWFA domain with the alphaV beta­propeller structure (4). The alphaV subunit contributes a "thigh and a calf" region, while the beta3 subunit contains a PSI domain and four cysteine­rich I­EGF folds. The alphaV subunit domains termed thigh, calf­1 and calf­2 generate a "knee" region that is bent when the alphaV beta3 is in its constitutively inactive state. Activation, either by "inside out" signaling or by Mg2+ or Mn2+ binding, extends the Integrin to expose its ligand binding site (1, 4). Within the extracellular domain (ECD), cynomolgus alphaV shares 99% amino acid (aa) sequence identity with the 962 aa human alphaV, while cynomolgus beta3 ECD shares almost 100% aa sequence identity with the 692 aa human beta3 ECD. Two splice variants of beta3 (b and c) diverge over the last 21 amino acids (aa) and lack cytoplasmic phosphorylation sites (5, 6). Another beta3 splice variant diverges after the vWFA domain, producing a soluble 60 kDa form in platelets and endothelial cells (7). alphaV beta3 is essential for the maturation of osteoclasts and their binding and resorption of bone, as well as promotes their apoptosis (8, 9). M­CSF R and alphaV beta3 share signaling pathways during osteoclastogenesis, and deletion of either molecule causes osteopetrosis (8, 9). Cell entry of several viruses is mediated by alphaV beta3 (4, 10). alphaV beta3 is involved in several other signaling pathways by direct interaction with receptor tyrosine kinases and ligands. For example, it cooperates with endothelial cell VEGF R2 in angiogenesis, and with IGF­1 to promote cancer cell proliferation and invasiveness (11, 12).

References

  1. Hynes, R. O. (2002) Cell 110:673.
  2. Serini, G. et al. (2006) Exp. Cell Res. 312:651.
  3. Ross, F. P. and S. L. Teitelbaum (2005) Immunol. Rev. 208:88.
  4. Xiong, J. et al. (2001) Science 294:339.
  5. Kumar, C. S. et al. (1987) J. Biol. Chem. 272:16390.
  6. vanKuppevelt, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5415.
  7. Djaffar, I. et al. (1994) Biochem. J. 300:69.
  8. McHugh, K. P. et al. (2000) J. Clin. Invest. 105:433.
  9. Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
  10. Chu, J. J. and M. Ng (2004) J. Biol. Chem. 279:54533.
  11. Somanath, P.R. et al. (2009) Angiogenesis 12:177.
  12. Saegusa, J. et al. (2009) J. Biol. Chem. 284:24106.

Alternate Names

antigen identified by monoclonal L230, CD51, CD51 antigen, integrin alpha-V, integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51), MSK8, Vitronectin receptor subunit alpha, VNRADKFZp686A08142

Entrez Gene IDs

3685 (Human)

Gene Symbol

ITGAV

UniProt

XP_005573729.1 (Integrin alphaV) & XP_005584667.1 (Integrin beta3)

Additional Integrin alpha V beta 3 Products

Product Documents for Recombinant Cynomolgus Integrin alpha V beta 3 Protein, CF

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