Recombinant Cynomolgus LIFR alpha Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10528-LR

R&D Systems, part of Bio-Techne
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Key Product Details

Source

HEK293

Accession #

XP_005556818.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all LIFR alpha Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey LIFR alpha protein
Cynomolgus Monkey LIFR alpha
(Gln45-Ser833)
Accession # XP_005556818.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln45 (blocked)

Predicted Molecular Mass

116 kDa

SDS-PAGE

133-149 kDa, under reducing conditions

Activity

Measured by its ability to inhibit LIF-dependent proliferation of TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
The ED50 for this effect is typically 0.7-7 μg/mL in the presence of 0.3 ng/mL of recombinant human LIF.

Scientific Data Images for Recombinant Cynomolgus LIFR alpha Fc Chimera Protein, CF

Recombinant Cynomolgus LIFR alpha Fc Chimera Protein Bioactivity

Recombinant Cynomolgus LIFR alpha Fc Chimera Protein Bioactivity

Measured by its ability to inhibit LIF-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.7-7 μg/mL in the presence of 0.3 ng/mL of recombinant Human LIF.
Recombinant Cynomolgus LIFR alpha Fc Chimera Protein SDS-PAGE

Recombinant Cynomolgus LIFR alpha Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey LIFR alpha Fc Chimera Protein (10528-LR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 133-149 kDa and 260-280 kDa, respectively.

Formulation, Preparation and Storage

10528-LR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LIFR alpha

Leukemia Inhibitory Factor Receptor alpha (LIF R alpha), also known as LIFR beta and CD118, is a 190 kDa type I transmembrane protein in the Interleukin-6 receptor family. Members of this family mediate the biological effects of Cardiotrophin-1, CLC, CNTF, IL-6, IL-11, IL-27, and Oncostatin M (1). Based on its similarity with human LIF R alpha,  mature cynomolgus LIF R alpha is predicted to consist of a 789 amino acid (aa) extracellular domain (ECD) with two cytokine receptor homology domains, one WSxWS motif, and three fibronectin type III repeats, followed by a 26 aa transmembrane segment and a 238 aa cytoplasmic domain (2). Within the ECD, cynomolgus LIF R alpha shares 97% aa sequence identity with human LIF R alpha. LIF R alpha binds the pleiotropic cytokine LIF with low affinity (3). Binding affinity is increased by the ligand-induced association of LIF R alpha with the signal transducing subunit gp130 (4, 6). The LIF R alpha/gp130 receptor complex also transduces Oncostatin M signals, although LIF R alpha alone does not interact with Oncostatin M (4). gp130 associates with different ligand-specific receptors to form signaling receptor complexes for the other IL-6 family ligands (1). The CNTF receptor is a ternary complex that contains CNTF R alpha and gp130 as well as LIF R alpha (6, 7). LIF R alpha is widely expressed, and LIF induces the proliferation, differentiation, and activation of cells in many tissues (8, 9). In particular, LIF R alpha plays an important role in several aspects of early pregnancy such as blastocyst implantation in the uterus (10-12).

References

  1. Muller-Newen, G. (2003) Science STKE 2003:pe40.
  2. Gearing, D.P. et al. (1991) EMBO J. 10:2839.
  3. Layton, M.J. et al. (1992) Proc. Natl. Acad. Sci. 89:8616.
  4. Gearing, D.P. et al. (1992) Science 255:1434.
  5. Giese, B. et al. (2005) J. Cell Sci. 118:5129.
  6. Ip, N.Y. et al. (1992) Cell 69:1121.
  7. Davis, S. et al. (1993) Science 260:1805.
  8. Metcalf, D (2003) Stem Cells 21:5.
  9. Kubota, Y. et al. (2008) J. Clin. Invest. 118:2393.
  10. Paiva, P. et al. (2009) Cytokine Growth Factor Rev. 20:319.
  11. Stewart, C.L. et al. (1992) Nature 359:76.
  12. Cheng, J.-G. et al. (2001) Proc. Natl. Acad. Sci. 98:8680.

Long Name

Leukemia Inhibitory Factor Receptor alpha

Alternate Names

CD118, LIF R alpha, LIF R beta, LIFR, LIFRa

Entrez Gene IDs

3977 (Human); 16880 (Mouse); 81680 (Rat); 102131906 (Cynomolgus Monkey)

Gene Symbol

LIFR

UniProt

XP_005556818.1

Additional LIFR alpha Products

Product Documents for Recombinant Cynomolgus LIFR alpha Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Cynomolgus LIFR alpha Fc Chimera Protein, CF

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