Recombinant Cynomolgus/Rhesus Macaque B7-1/CD80 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9695-B1

Fc Chimera
R&D Systems, part of Bio-Techne
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Key Product Details

Source

HEK293

Accession #

XP_005548122

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all B7-1/CD80 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived B7-1/CD80 protein
Cynomolgus Monkey B7-1/CD80
(Val35-Asn242)
Accession # XP_005548122		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val35

Predicted Molecular Mass

51 kDa

SDS-PAGE

53-79 kDa, reducing conditions

Activity

Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells. Freeman, G.J. et al. (1993) Science 262:909.
The ED50 for this effect is 0.75-4.5 μg/mL.

Scientific Data Images for Recombinant Cynomolgus/Rhesus Macaque B7-1/CD80 Protein, CF

Recombinant Cynomolgus/Rhesus Macaque B7-1/CD80 Protein SDS-PAGE

Recombinant Cynomolgus/Rhesus Macaque B7-1/CD80 Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey B7‑1/CD80 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 53-79 kDa and 105-160 kDa, respectively.

Formulation, Preparation and Storage

9695-B1
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution
Reconstitute at 250 μg/mL in water.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: B7-1/CD80

B7-1/CD80 and B7-2/CD86, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses (1). Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100-fold higher affinity than CD28 and is involved in the down-regulation of the immune response (2-5). B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells (2). Mature cynomolgus monkey B7-1 consists of a 213 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 22 aa transmembrane domain, and a 20 aa cytoplasmic domain. Within the ECD, cynomolgus monkey B7-1 shares 97% and 51% aa sequence identity with human and mouse B7-1, respectively. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas (6).

References

  1. Ville, S. et al. (2015) Front. Immunol. 6:411.
  2. Azuma, M. et al. (1993) Nature 366:76.
  3. Freeman, G.J. et al. (1993) Science 262:909.
  4. Chen, C. et al. (1994) J. Immunol. 152:4929.
  5. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
  6. Mir, M.A., et al. (2008) Expert Opin. Ther. Targets 12:969.

Alternate Names

B71, CD80

Entrez Gene IDs

941 (Human); 12519 (Mouse); 25408 (Rat); 102140078 (Cynomolgus Monkey)

Gene Symbol

CD80

UniProt

XP_005548122

Additional B7-1/CD80 Products

Product Documents for Recombinant Cynomolgus/Rhesus Macaque B7-1/CD80 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Cynomolgus/Rhesus Macaque B7-1/CD80 Protein, CF

For research use only

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