Recombinant Equine PDGF-BB Protein

R&D Systems, part of Bio-Techne | Catalog # 8585-BB

R&D Systems, part of Bio-Techne
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Carrier Free
8585-BB-010/CF
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With Carrier
8585-BB-010
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Key Product Details

Source

E. coli

Accession #

XP_005606703

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all PDGF-BB Proteins and Enzymes »

Product Specifications

Source

E. coli-derived equine PDGF-BB protein
Ser51-Thr159

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser51

Predicted Molecular Mass

12 kDa

SDS-PAGE

12 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749.
The ED50 for this effect is 5-30 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 8585-BB
Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 8585-BB/CF
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF-BB

Platelet-Derived Growth Factor (PDGF)-BB is synthesized as a prepro-precurser that contains a signal peptide, an N-terminal prodomain, a mature region, and a
C-terminal prodomain (1-4). The proprecursor is initially dimerized and then intracellulary processed twice. The N-terminal prodomain is cleaved first, followed by cleavage of the C-terminal prodomain prior to the formation of a disulfide-linked homodimer. Mature equine PDGF-B shares 95%, 88%, 89%, 83%, 79%, and 79% amino acid sequence identity with human, mouse, rat, sheep, cow, and goat PDGF-B, respectively. PDGF-BB is expressed by hepatocytes and nonresorbing osteoclasts, generating osteoblasts and bone formation (4, 5). It is also produced by platelets, macrophages, and mast cells. At sites of injury, it promotes neutrophil and macrophage infiltration for debridement, fibroblast secretion of new extracellular matrix, and IGF-I-mediated re-epithelialization (6, 7). The traditional receptor for PDGF is either a homodimer or heterodimer created from two type I transmembrane RTKs, PDGF R alpha and PDGF R beta (8, 9). PDGF-BB has been shown to bind the alpha alpha homodimer, alpha beta heterodimer, and the beta beta homodimer in vitro, and act through the beta beta homodimer in vivo (8, 10). Equine PDGF-BB has been shown to stimulate contraction of fibroblast-populated collagen matrix, a model of equine wound contraction, in a Rho kinase- and p38-dependent manner (12). PDGF-BB is present in platelet-rich plasma (PRP), which has been used to treat soft tissue injuries in horses (13, 14). The levels of equine PDGF-BB in PRP is dependent on breed, gender, and age (15).

References

  1. Rao, C.D. et al. (1986) Proc. Natl. Acad. Sci. USA 83:2392.
  2. Kaetzel, D.M. et al. (1996) Biochim. Biophys. Acta. 1298:250.
  3. Ostman, A. et al. (1992) J. Cell Biol. 118:509.
  4. Siegfried, G. et al. (2005) Oncogene 24:6925.
  5. Kreja, L. et al. (2010) J. Cell. Biochem. 109:347.
  6. van Steensel, L. et al. (2012) J. Clin. Endocrinol. Metab. 97:E400.
  7. Barrientos, S. et al. (2008) Wound Repair Regen. 16:585.
  8. Andrae, J. et al. (2008) Genes Dev. 22:1276.
  9. Heldin, C.H. and B. Westermark (1999) Physiol. Rev. 79:1283.
  10. Li, X. and U. Eriksson (2003) Cytokine Growth Factor Rev. 14:91.
  11. Watts, E.J. and M.T. Rose (2010) Domest. Anim. Endocrinol. 38:253.
  12. Boswell, S.G. et al. (2014) Am. J. Sports Med. 42:42.
  13. Halper, J. (2014) Adv. Exp. Med. Biol. 802:59.
  14. Giraldo, C.E. et al. (2013) BMC Vet. Res. 9:29.

Long Name

Platelet-derived Growth Factor BB

Alternate Names

PDGFBB

Entrez Gene IDs

5155 (Human); 24628 (Rat)

Gene Symbol

PDGFB

UniProt

XP_005606703

Additional PDGF-BB Products

Product Documents for Recombinant Equine PDGF-BB Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 8585-BB (with carrier)
Download SDS 8585-BB/CF (carrier free)

Product Specific Notices for Recombinant Equine PDGF-BB Protein

For research use only

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