Recombinant Feline IL-15 Protein

R&D Systems, part of Bio-Techne | Catalog # 7805-FL

R&D Systems, part of Bio-Techne
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Carrier Free
7805-FL-010/CF
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With Carrier
7805-FL-010
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Key Product Details

Source

E. coli

Accession #

O97687

Structure / Form

Monomer

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-15 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived feline IL-15 protein
Asn49-Ser162

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asn49

Predicted Molecular Mass

13 kDa

SDS-PAGE

10 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359.
The ED50 for this effect is 0.2-1.2 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 7805-FL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 7805-FL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-15

Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases (1). Mature feline IL-15 shares 80%, 83%, 68%, and 68% amino acid sequence identity with canine, human, mouse, and rat IL-15, respectively (2). IL-15 binds with high affinity to IL-15 R alpha (3). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gammac) which are also subunits of the IL-2 receptor complex (4). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (5). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta/ gammac on adjacent cells (6). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (5). In human and mouse, soluble IL-15-binding forms of IL‑15 R alpha can be generated by proteolytic shedding and bind up nearly all the IL-15 in circulation (7‑9). Soluble IL-15 R alpha functions as an inhibitor that limits IL-15 action (3, 8). Ligation of membrane-associated IL-15/IL-15 R alpha complexes also induces reverse signaling that promotes activation of the IL-15/IL-15 R alpha expressing cells (10). IL-15 induces or enhances the differentiation, maintenance, or activation of multiple T cell subsets including NK, NKT, Th17, Treg, and CD8+ memory cells (11‑15). An important component of these functions is the ability of IL-15 to induce dendritic cell differentiation and inflammatory activation (10, 13). IL-15 exhibits anti-tumor activity independent of its actions on NK cells or CD8+ T cells (16). It also inhibits the deposition of lipid in adipocytes, and its circulating levels are decreased in obesity (17).

References

  1. De Sabatino, A. et al. (2011) Cytokine Growth Factor Rev. 22:19.
  2. Dean, G.A. et al. (2005) Cytokine 29:77.
  3. Giri, J.G. et al. (1995) EMBO J. 14:3654.
  4. Giri, J. et al. (1994) EMBO J. 13:2822.
  5. Dubois, S. et al. (2002) Immunity 17:537.
  6. Castillo, E.F. and K.S. Schluns (2012) Cytokine 59:479.
  7. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
  8. Mortier, E. et al. (2004) J. Immunol. 173:1681.
  9. Bergamaschi, C. et al. (2012) Blood 120:e1.
  10. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
  11. Mortier, E. et al. (2008) J. Exp. Med. 205:1213.
  12. Gordy, L.E. et al. (2011) J. Immunol. 187:6335.
  13. Harris, K.M. (2011) J. Leukoc. Biol. 90:727.
  14. Xia, J. et al. (2010) Clin. Immunol. 134:130.
  15. Schluns, K.S. et al. (2002) J. Immunol. 168:4827.
  16. Davies, E. et al. (2010) J. Leukoc. Biol. 88:529.
  17. Barra, N.G. et al. (2010) Obesity 18:1601.

Long Name

Interleukin 15

Alternate Names

IL15

Entrez Gene IDs

3600 (Human); 16168 (Mouse); 25670 (Rat); 102119613 (Cynomolgus Monkey); 493682 (Feline)

Gene Symbol

IL15

UniProt

O97687

Additional IL-15 Products

Product Documents for Recombinant Feline IL-15 Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 7805-FL (with carrier)
Download SDS 7805-FL/CF (carrier free)

Product Specific Notices for Recombinant Feline IL-15 Protein

For research use only

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