Recombinant HCoV-NL63 Spike RBD His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10605-CV

R&D Systems, part of Bio-Techne
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10605-CV-100
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Key Product Details

Source

HEK293

Accession #

YP_003767.1

Conjugate

Unconjugated

Applications

Bioactivity

View all Spike RBD Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived hcov-nl63 Spike RBD protein
Ala475-Asp634, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala475 & Leu476

Predicted Molecular Mass

19 kDa

SDS-PAGE

30-38 kDa, under reducing conditions

Activity

Recombinant HCoV-NL63 Spike RBD His-tag Protein (Catalog # 10605-CV) binds Recombinant Human ACE-2 Fc Chimera Protein  (Catalog # 10544-ZN) in a functional ELISA.

Scientific Data Images for Recombinant HCoV-NL63 Spike RBD His-tag Protein, CF

Recombinant HCoV-NL63 Spike RBD His-tag Protein Binding Activity.

Recombinant HCoV-NL63 Spike RBD His-tag Protein (Catalog # 10605-CV) binds Recombinant Human ACE-2 Fc Chimera Protein (10544-ZN) in a functional ELISA.

Recombinant HCoV-NL63 Spike RBD His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant HCoV-NL63 Spike RBD His-tag (Catalog # 10605-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 30-38 kDa.

Formulation, Preparation and Storage

10605-CV
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Spike RBD

HCoV-NL63, a virus first isolated from a child suffering from respiratory disease in 2003, belongs to a family of viruses known as coronaviruses that are commonly comprised of a large plus-strand RNA genome and four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1, 2). Other well-known human coronaviruses include three viruses that cause relatively mild respiratory disease: HCoV-229E, HCoV-HKU1 and HCov-OC43, plus three viruses that cause the Severe Acute Respiratory Syndrome (SARS-CoV), the Middle East Respirator Syndrome (MERS-CoV), and the global pandemic Covid-19 (SARS-CoV2). HCov-NL63 Spike Protein (S Protein) is a glycoprotein that mediates membrane fusion and viral entry. As with most coronaviruses, proteolytic cleavage of the S protein generates two distinct peptides, S1 and S2 subunits. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion.  Although HCoV-NL63 S protein shares high homology (56%) with HCoV-229E, it does not employ CD13 (aminopeptidase N) as the receptor like HCoV-229E. Instead, HCoV-NL63 engages Angiotensin-Converting Enzyme 2 (ACE-2), the same receptor as SARS-CoV and SARS-CoV2, for cellular entry and replication (3). The receptor binding domain (RBD) of HCoV-NL63 is located at C-terminal region of S1 subunit (4, 5). Although NL63-CoV and SARS-CoV do not share structural homology in RBD region, they bind an overlapping region of ACE-2 (6, 7).

References

  1. Van der Hoek, L. et al. (2004) Nat. Med. 10:368.
  2. Fouchier, R.M. et al. (2004) Proc. Natl. Acad. Sci. U.S.A. 101:6212.
  3. Hofmann, H. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102:7988.
  4. Hofmann, H. et al. (2006) J. Virol. 80:8639.
  5. Lin, H. et al. (2008) J. Gen. Virol. 89:1015.
  6. Li, W. et al. (2007) Virology 367:367.
  7. Wu, K. et al. (2009) Proc. Natl. Acad. Sci. U.S.A. 106:19970.

Long Name

Spike Receptor Binding Domain

Entrez Gene IDs

3200426 (HCoV-HKU1); 14254594 (MERS-CoV); 1489668 (SARS-CoV); 43740568 (SARS-CoV-2)

Gene Symbol

S

UniProt

YP_003767.1

Additional Spike RBD Products

Product Documents for Recombinant HCoV-NL63 Spike RBD His-tag Protein, CF

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Product Specific Notices for Recombinant HCoV-NL63 Spike RBD His-tag Protein, CF

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