Recombinant Human Active MAP3K8/Tpl2/COT Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 4586-KS

R&D Systems, part of Bio-Techne
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4586-KS-010
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Key Product Details

Source

Sf 9 (baculovirus)

Accession #

NM_005204

Conjugate

Unconjugated

Applications

Bioactivity

View all MAP3K8/Tpl2/COT Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 9 (baculovirus)-derived human MAP3K8/Tpl2/COT protein
aa 30-397

Purity

>75%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

N-terminal Sequence Analysis

Using an N-terminal GST tag

SDS-PAGE

70 kDa

Activity

The specific activity of COT is typically 860-1164 nmol/min/mg using MEK1 and ERK1 substrates and a myelin basic protein (MBP) substrate.

Scientific Data Images for Recombinant Human Active MAP3K8/Tpl2/COT Protein, CF

Recombinant Human Active MAP3K8/Tpl2/COT Protein SDS-PAGE.

Recombinant Human Active MAP3K8/Tpl2/COT Protein SDS-PAGE

The approximate molecular weight is 70 kDa and the purity is > 75%.

Formulation, Preparation and Storage

4586-KS
Formulation Supplied in 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 10 mM Glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, and 25% Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage This product is stable at ≤ ‑70 °C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.

Background: MAP3K8/Tpl2/COT

COT is an oncogene that can activate both the MAP kinase and JNK kinase pathways. COT activates I kappaB kinases and induces the nuclear production of NF-kappa B. The C-terminal catalytic domain of KSR2 associates with COT and KSR2 can negatively regulate the kinase activity of COT in vitro. Co-transfection of KSR2 with COT in cells lead to reduced COT-mediated ERK activation and COT induced IL-8 production in a dose-dependent manner (1). COT is one of the MAP kinase kinase (MAPKK) kinases that regulates the ERK1/ERK2 pathway in response to IL-1. Blockage of expression of COT results in failure of IL-1 to induce an increase in IL 8 and MIP-1 beta mRNA levels (2).

References

  1. Channavajhala, P.L. et al. (2003) J. Biol. Chem. 278:47089.
  2. Rodriguez, C. et al. (2006) Cell Signal. 18:1376.

Long Name

Mitogen-activated Protein Kinase Kinase Kinase 8

Alternate Names

COT, EST, ESTF, MAP3K8, Tpl-2

Entrez Gene IDs

1326 (Human); 26410 (Mouse); 116596 (Rat)

Gene Symbol

MAP3K8

UniProt

NM_005204 (Human)

Additional MAP3K8/Tpl2/COT Products

Product Documents for Recombinant Human Active MAP3K8/Tpl2/COT Protein, CF

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Active MAP3K8/Tpl2/COT Protein, CF

For research use only

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