Recombinant Human Angiopoietin-like 4 C-Terminal Frag, CF

R&D Systems, part of Bio-Techne | Catalog # 3485-AN

R&D Systems, part of Bio-Techne
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3485-AN-050
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Key Product Details

Source

NS0

Accession #

Q9BY76

Structure / Form

Oligomer

Conjugate

Unconjugated

Applications

Inhibition Activity

View all Angiopoietin-like Protein 4/ANGPTL4 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Angiopoietin-like Protein 4/ANGPTL4 protein
Leu165-Ser406, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu165

Predicted Molecular Mass

28 kDa

SDS-PAGE

36 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Angiopoietin‑like 4/ANGPTL4 C‑Terminal Fragment is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera  (Catalog # 2078-T4) binds with an ED50 of 70.0-350 ng/mL.

Formulation, Preparation and Storage

3485-AN
Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitution
Reconstitute at 250 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 4/ANGPTL4

ANGPT-L4, also known as FIAF, FARP, and PGAR, is a 55 kDa glycoprotein secreted by the liver and fat tissue that is structurally related to the angiopoietins. It contains an N‑terminal coiled coil domain and a C‑terminal fibrinogen-like domain which can be proteolytically separated in vivo (1, 2). Mature human ANGPT‑L4 shares 26%‑30% amino acid (aa) sequence identity with ANGPT-L1, 2, 3, 5, 6, and 7. It shares approximately 75% aa sequence identity with mouse and rat ANGPT-L4. The coiled coil domain, which is not glycosylated, mediates the formation of variable sized disulfide-linked oligomers (3, 4). This domain directly inhibits lipoprotein lipase, resulting in increased circulating triglyceride levels (5-9). In human, the N-terminal fragment and full length ANGPT-L4 physically associate with HDL (9). In mouse, however, full length ANGPT-L4 associates with HDL, while the N-terminal fragment associates with LDL (9). Circulating ANGPT-L4 is decreased in type II diabetics with a subsequent loss of its normal plasma glucose lowering activity (10). Its expression in adipose tissue is induced by fasting and suppressed by feeding (3, 11). Its expression in both liver and fat is up‑regulated by PPAR alpha, beta, gamma, and delta agonists and down‑regulated by insulin (3, 12, 13). ANGPT-L4 is induced in vascular endothelial cells by hypoxia and in hypoxic areas surrounding tumors (14-16). The full length molecule but not the C-terminal fragment is bound by heparan sulfate proteoglycans and functions as an angiogenesis inhibitor (14, 15).

References

  1. Li, C. (2006) Curr. Opin. Lipidol. 17:152.
  2. Kersten, S. (2005) Biochem. Soc. Transact. 33:1059.
  3. Ge, H. et al. (2005) J. Lipid Res. 46:1484.
  4. Ge, H. et al. (2004) J. Biol. Chem. 279:2038.
  5. Sukonina, V. et al. (2006) Proc. Natl. Acad. Sci. 103:17450.
  6. Koster, A. et al. (2005) Endocrinology 146:4943.
  7. Yoshida, K. et al. (2002) J. Lipid Res. 43:1770.
  8. Ge, H. et al. (2004) J. Lipid Res. 45:2071.
  9. Mandard, S. et al. (2006) J. Biol. Chem. 281:934.
  10. Xu, A. et al. (2005) Proc. Natl. Acad. Sci. 102:6086.
  11. Kersten, S. et al. (2000) J. Biol. Chem. 275:28488.
  12. Mandard, S. et al. (2004) J. Biol. Chem. 279:34411.
  13. Yamada, T. et al. (2006) Biochem. Biophys. Res. Commun. 347:1138.
  14. Cazes, A. et al. (2006) Circ. Res. 99:1207.
  15. Le Jan, S. et al. (2003) Am. J. Pathol. 162:1521.
  16. Ito, Y. et al. (2003) Cancer Res. 63:6651.

Alternate Names

Angiopoietin like Protein 4, ANGPTL4, FIAF, HFARP, PGAR

Entrez Gene IDs

51129 (Human); 57875 (Mouse); 362850 (Rat)

Gene Symbol

ANGPTL4

UniProt

Q9BY76

Additional Angiopoietin-like Protein 4/ANGPTL4 Products

Product Documents for Recombinant Human Angiopoietin-like 4 C-Terminal Frag, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Angiopoietin-like 4 C-Terminal Frag, CF

For research use only

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