Recombinant Human B7-1/CD80 His-tag Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 9050-B1
Key Product Details
Product Specifications
Source
Human embryonic kidney cell, HEK293-derived human B7-1/CD80 protein
Val35-Asn242, with a C-terminal 6-His tag
Val35-Asn242, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Val35
Predicted Molecular Mass
25 kDa
SDS-PAGE
45-58 kDa, reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
When Recombinant Human B7-1/CD80 is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human CTLA-4 Fc Chimera (Catalog # 7268-CT) that produces 50% of the optimal binding response is approximately 0.05-0.3 μg/mL.
When Recombinant Human B7-1/CD80 is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human CTLA-4 Fc Chimera (Catalog # 7268-CT) that produces 50% of the optimal binding response is approximately 0.05-0.3 μg/mL.
Reviewed Applications
Read 1 review rated 4 using 9050-B1 in the following applications:
Formulation, Preparation and Storage
9050-B1
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 500 μg/mL in PBS.
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| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: B7-1/CD80
B7-1. Alternative splicing generates a 30 kDa soluble isoform that lacks the transmembrane segment and retains the ability to bind CD28 and CTLA-4 and an isoform that lacks the second Ig-like domain and the transmembrane segment (8). Both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and
CTLA-4 (1). B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells (2).
References
- Ville, S. et al. (2015) Front. Immunol. 6:411.
- Azuma, M. et al. (1993) Nature 366:76.
- Freeman, G.J. et al. (1993) Science 262:909.
- Chen, C. et al. (1994) J. Immunol. 152:4929.
- Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
- Lanier, L. et al. (1995) J. Immunol. 154:97.
- Freeman, G.J. et al. (1989) J. Immunol. 143:2714.
- Kakoulidou, M. et al. (2007) Scand. J. Immunol. 66:529.
Alternate Names
B71, CD80
Gene Symbol
CD80
UniProt
Additional B7-1/CD80 Products
Product Documents for Recombinant Human B7-1/CD80 His-tag Protein, CF
Product Specific Notices for Recombinant Human B7-1/CD80 His-tag Protein, CF
For research use only
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