Recombinant Human B7-H2 Isoform 3 Fc Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI10028

Biotinylated
R&D Systems, part of Bio-Techne
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

HEK293

Accession #

NP_001269980.1

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all B7-H2 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human B7-H2 protein
Human B7-H2
(Ala19-Thr139)
Accession # NP_001269980.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala19

Predicted Molecular Mass

42 kDa

SDS-PAGE

61-70 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human ICOS Fc Chimera (Catalog # 169-CS) is immobilized at 10 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant B7-H2 Isoform 3 Fc Chimera Avi-tag (Catalog # AVI10028) that produces 50% of the optimal binding response is approximately 10-50 μg/mL.

Scientific Data Images for Recombinant Human B7-H2 Isoform 3 Fc Avi-tag Protein, CF

Recombinant Human B7-H2 Isoform 3 Fc Avi-tag Protein Binding Activity

Recombinant Human B7-H2 Isoform 3 Fc Avi-tag Protein Binding Activity

When Recombinant Human ICOS Fc Chimera (Catalog # 169-CS) is immobilized at 10 µg/mL, 100 µL/well, the concentration of Biotinylated Recombinant B7-H2 Isoform 3 Fc Chimera Avi-tag (Catalog # AVI10028) that produces 50% of the optimal binding response is approximately 10-50 µg/mL.
Recombinant Human B7-H2 Isoform 3 Fc Avi-tag Protein SDS-PAGE

Recombinant Human B7-H2 Isoform 3 Fc Avi-tag Protein SDS-PAGE

2 μg/lane of Biotinylated Recombinant Human B7-H2 Isoform 3 Fc Chimera Avi-tag (Catalog # AVI10028) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 61-70 kDa and 120-140 kDa.

Formulation, Preparation and Storage

AVI10028
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-H2

B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules (1). Mature human B7-H2 consists of a 238 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like (Ig-like) domains, a 21 aa transmembrane segment, and a 25 aa cytoplasmic domain (2, 3). Within the ECD, human B7-H2 shares 50% and 54% aa sequence identity with mouse and rat B7‑H2, respectively. Alternative splicing generates a long isoform (Isoform 2),  which carries a 10 aa substitution for the C-terminal 3 residues, and a short isoform (Isoform 3; this product), which lacks the first Ig-like domain (aa 18 - 134 is missing) . B7-H2 is expressed on antigen presenting cells such as B cells, macrophages, monocytes, and dendritic cells (2-6). It binds to ICOS on activated T cells, leading to both positive and negative effects on immune responses including its own down-regulation (2, 4, 7). Mouse and human B7-H2 exhibit cross-species binding to ICOS (3, 6). The B7-H2 interaction with ICOS is costimulatory for T cell proliferation as well as the development of B cells, plasma cells, follicular helper T cells (TFH) and germinal centers (2-4, 8, 9). In human but not in mouse, B7-H2 additionally binds to CD28 and CTLA4, and its interaction with CD28 can costimulate both human and mouse naïve T cells and regulatory T cells (Treg) (6). B7-H2 contributes to the development of allergic asthma by enhancing Th2 biased immune responses, limiting Th17 responses, and promoting eosinophilic infiltration into the lung (8, 10, 11). Its activation of ICOS on Treg limits pulmonary inflammation and airway hyperresponsiveness, promotes the development of inhalational tolerance, and impairs anti-tumor immunity (5, 12, 13). In contrast, its ligation of ICOS on TFH cells can increase the severity of autoimmune symptoms (9). A soluble form of human B7-H2 is elevated in the circulation of patients with active systemic lupus erythematosus (14). In the thyroid, B7-H2 is up-regulated on thyrocytes during inflammation and promotes their proliferation and production of thryoid hormones (15).

References

  1. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
  2. Wang, S. et al. (2000) Blood 96:2808.
  3. Yoshinaga, S.K. et al. (2000) Int. Immunol. 12:1439.
  4. Yoshinaga, S.K. et al. (1999) Nature 402:827.
  5. Faget, J. et al. (2012) Cancer Res. 72:6130.
  6. Yao, S. et al. (2011) Immunity 32:729.
  7. Watanabe, M. et al. (2008) J. Immunol. 180:5222.
  8. Wong, S.-C. et al. (2003) Blood 102:1381.
  9. Hu, Y.-L. et al. (2009) J. Immunol. 182:1421.
  10. Kadkhoda, K. et al. (2010) J. Immunol. 184:3780.
  11. Kadkhoda, K. et al. (2011) Int. Immunol. 23:239.
  12. Gajewska, B.U. et al. (2005) J. Immunol. 174:3000.
  13. Akbari, O. et al. (2002) Nat. Med. 8:1024.
  14. Her, M. et al. (2009) Lupus 18:501.
  15. Wang, F. et al. (2012) J. Clin. Immunol. 32:1253.

Long Name

B7 Homolog 2

Alternate Names

B7H2, B7RP-1, CD275, GL50, ICOSL, ICOSLG

Entrez Gene IDs

23308 (Human); 50723 (Mouse); 499415 (Rat); 102132485 (Cynomolgus Monkey)

Gene Symbol

ICOSLG

UniProt

NP_001269980.1

Additional B7-H2 Products

Product Documents for Recombinant Human B7-H2 Isoform 3 Fc Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human B7-H2 Isoform 3 Fc Avi-tag Protein, CF

For research use only

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