Recombinant Human BACE-1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 931-AS
Key Product Details
Source
NS0
Accession #
Structure / Form
Pro and Mature forms
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human BACE-1 protein
Thr22-Tyr460 (pro) & Glu46-Tyr460 (mature), both with a C-terminal 10-His tag
Thr22-Tyr460 (pro) & Glu46-Tyr460 (mature), both with a C-terminal 10-His tag
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Thr22 & Glu46
Predicted Molecular Mass
50 kDa (Pro form) & 47 kDa (Mature form)
SDS-PAGE
68-70 kDa and 65-67 kDa, reducing conditions
Activity
Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK(Dpn)RR-NH2 (Catalog # ES004).
The specific activity is >3.5 pmol/min/µg, as measured under the described conditions.
The specific activity is >3.5 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
931-AS
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 500 μg/mL in sterile, deionized water.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: BACE-1
BACE-1 is an aspartic protease and an integral membrane protein (1-5). BACE-1 is the peptidase predominantly responsible for cleavage of the amyloid precursor protein beta site in the brain to generate the amyloid beta peptide. Because the amyloid beta peptide is a major component of amyloid plaques, BACE-1 has been implicated in the onset and/or progression of Alzheimer's disease. BACE-1 is expressed in a variety of human tissues. It is likely that this peptidase has functions in addition to its hydrolysis of the amyloid precursor protein. The peptidase activity of BACE-1 is optimal under mildly acidic conditions (pH 3.5-5.5), consistent with its proposed function in an acidic intracellular compartment.
References
- Ermolieff, J. et al. (2000) Biochemistry 39:12450.
- Lin, X. et al. (2000) Proc. Natl. Acad. Sci. USA 97:1456.
- Sinha, S. et al. (1999) Nature 402:537.
- Vassar, R. et al. (1999) Science 286:735.
- Yan, R. et al. (1999) Nature 402:533.
Long Name
beta-site Ayloid Precursor Protein Cleaving Enzyme 1
Alternate Names
BACE1
Gene Symbol
BACE1
UniProt
Additional BACE-1 Products
Product Documents for Recombinant Human BACE-1 Protein, CF
Product Specific Notices for Recombinant Human BACE-1 Protein, CF
For research use only
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