Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF Best Seller
R&D Systems, part of Bio-Techne | Catalog # 193-BC
Analyzed by SEC-MALS
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human BCMA/TNFRSF17 protein
| Human BCMA (Met1-Ala54) Accession # Q6PE46 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met1
Predicted Molecular Mass
32 kDa (monomer)
SDS-PAGE
35-45 kDa, reducing conditions
Activity
Measured by its ability to inhibit APRIL-mediated proliferation of anti-IgM stimulated mouse B cells. Moore, P.A. et al. (1999) Science 285:260; Gross, J.A. et al. (2000) Nature 404:995; Schneider, P. et al. (1999) J. Exp. Med. 189:1747.
The ED50 for this effect is 0.01-0.04 µg/mL in the presence of 100 ng/mL of rhAPRIL.
The ED50 for this effect is 0.01-0.04 µg/mL in the presence of 100 ng/mL of rhAPRIL.
Reviewed Applications
Read 3 reviews rated 5 using 193-BC in the following applications:
Scientific Data Images for Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF
Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein SEC-MALS.
Recombinant Human BCMA/TNFRSF17 Fc Chimera (Catalog # 193-BC) has a molecular weight (MW) of 70.6 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Bioactivity of Human BCMA / TNFRSF17 Protein
Recombinant human BCMA / TNFRSF17 (193-BC) inhibits APRIL-mediated proliferation of anti-IgM stimulated mouse B cells. The ED50 for this effect is 0.01-0.4 µg/mL in the presence of 100 ng/mL of recombinant human APRIL.Formulation, Preparation and Storage
193-BC
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
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| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: BCMA/TNFRSF17
BCMA, B cell maturation antigen, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Human BCMA is a 184 amino acid (aa) protein consisting of a 54 aa extracellular domain, a 23 aa transmembrane domain, and a 107 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity.
References
- Madry, C. et al. (1998) Int. Immunol. 10:1693.
- Gras, M. et al. (1995) Int. Immunol. 7:1093.
- Kwon, B. et al. (1999) Curr. Opin. Immunol. 11:340.
- Marsters, S. et al. (2000) Curr. Biol. 10:785.
- Thompson, J. et al. (2000) J. Exp. Med. 192:129.
Long Name
B Cell Maturation Factor
Alternate Names
CD269, TNFRSF13A, TNFRSF17
Gene Symbol
TNFRSF17
UniProt
Additional BCMA/TNFRSF17 Products
Product Documents for Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF
Product Specific Notices for Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF
For research use only
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