Recombinant Human BTLA Fc Chimera (aa 25-150) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8385-BT

R&D Systems, part of Bio-Techne
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8385-BT-050
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Key Product Details

Source

NS0

Accession #

AAP44003

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all BTLA Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human BTLA protein
Human BTLA
Ile25-Ser150
Accession # AAP44003		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>85%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile25

Predicted Molecular Mass

41 kDa

SDS-PAGE

53-65 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse HVEM/TNFRSF14 Fc Chimera (Catalog # 2516-HV) is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Human BTLA Fc Chimera that produces 50% of the optimal binding response is approximately 0.25-1.25 μg/mL.

Reviewed Applications

Read 1 review rated 5 using 8385-BT in the following applications:

Scientific Data Images for Recombinant Human BTLA Fc Chimera (aa 25-150) Protein, CF

Recombinant Human BTLA Fc Chimera (aa 25-150) Protein Binding Activity

Recombinant Human BTLA Fc Chimera (aa 25-150) Protein Binding Activity

When Recombinant Mouse HVEM/TNFRSF14 Fc Chimera (Catalog # 2516-HV) is immobilized at 0.5 µg/mL, Recombinant Human BTLA Fc Chimera binds with an ED50 of 0.25-1.25 µg/mL.

Formulation, Preparation and Storage

8385-BT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BTLA

B- and T-lymphocyte attenuator (BTLA; CD272) is a 35 kDa type I transmembrane glycoprotein in the CD28 family of T cell co-stimulatory molecules (1-3). Mature human BTLA contains a 127 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane sequence, and a 111 aa cytoplasmic domain. The two ITIM motifs and three Tyr phosphorylation sites in the cytoplasmic tail transmit inhibitory signaling (4-5). The ECD of human BTLA shares 42% and 44% aa identity with that of mouse and rat BTLA, respectively. A splice variant lacking the transmembrane domain has been reported (6). Unlike other CD28 family members, the BTLA Ig domain in the ECD is of the I-type rather than V-type, and BTLA does not form homodimers (7). BTLA is also unusual in its interaction with the TNF superfamily member HVEM rather than with B7 family ligands (8). BTLA is expressed on T cells, B cells, macrophages, dendritic cells, and NK cells (9). Its expression is low in naïve T cells and increases during antigen-specific induction of anergy. In B cells, BTLA expression is highest in mature naïve cells (9). BTLA apparently limits T cell numbers, since its deletion results in overproduction of T cells, especially CD8+ memory T cells that are hyper-responsive to TCR cross-linking (10). Under the control of ROR gammat and IL-7, BTLA regulates the homeostasis and inflammatory responses of gamma deltaT cells (11). The binding of BTLA and HVEM does not preclude the concurrent binding of other HVEM ligands such as LIGHT or Lymphotoxin-alpha (12).

References

  1. Murphy, K.M. et al. (2006) Nat. Rev. Immunol. 6:671.
  2. Croft, M. (2005) Trends. Immunol. 26:292.
  3. Watanabe, N. et al. (2003) Nat. Immunol. 4:670.
  4. Gavrieli, M. et al. (2003) Biochem. Biophys. Res. Commun. 312:1236.
  5. Chemnitz, J.M. et al. (2006) J. Immunol. 176:6603.
  6. Han, P. et al. (2004) J. Immunol. 172 :5931.
  7. Compaan, D.M. et al. (2005) J. Biol. Chem. 280:39553.
  8. Sedy, J. R. et al. (2005) Nat. Immunol. 6:90.
  9. Hurchla, M.A. et al. (2005) J. Immunol. 174:3377.
  10. Krieg, C. et al. (2007) Nat. Immunol. 8:162.
  11. Bekiaris, V. et al. (2013) Immunity 39:1082.
  12. Cai G and Freeman GJ, (2009) Immunol Rev. 229:244

Long Name

B- And T-Lymphocyte Attenuator

Alternate Names

CD272

Entrez Gene IDs

151888 (Human); 208154 (Mouse); 102144877 (Cynomolgus Monkey)

Gene Symbol

BTLA

UniProt

AAP44003

Additional BTLA Products

Product Documents for Recombinant Human BTLA Fc Chimera (aa 25-150) Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human BTLA Fc Chimera (aa 25-150) Protein, CF

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