Recombinant Mouse HVEM/TNFRSF14 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2516-HV

R&D Systems, part of Bio-Techne
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2516-HV-100
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Key Product Details

Source

CHO

Accession #

NP_849262

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

View all HVEM/TNFRSF14 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse HVEM/TNFRSF14 protein
Mouse HVEM
(Gln39-Val207)
Accession # NP_849262		 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln39

Predicted Molecular Mass

45.6 kDa

SDS-PAGE

60-65 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse (rm) HVEM Fc Chimera is immobilized at 100 ng/ mL, 100 μL/well, the concentration of biotinylated rmBTLA Fc Chimera that produces 50% of the optimal binding response is found to be approximately 30-120 ng/mL.

Formulation, Preparation and Storage

2516-HV
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 1 mg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HVEM/TNFRSF14

HVEM (herpesvirus entry mediator), also known as TNFRSF14 and CD270, is a type I membrane protein in the TNF receptor superfamily, and it can both promote and inhibit T cell activity (1). Mature mouse HVEM consists of a 170 amino acid (aa) extracellular domain (ECD) with three cysteine-rich domains (CRD), a 24 aa transmembrane segment, and a 45 aa cytoplasmic tail with a TRAF interaction domain (2). Within the ECD, mouse HVEM shares 55% and 89% aa sequence identity with human and rat HVEM, respectively. HVEM is highly expressed on naïve CD4+ T cells, CD8+ T memory cells, regulatory T cells, dendritic cells, monocytes, and neutrophils (3-7). Its expression declines during effector T cell activation but is upregulated during Treg activation (3, 4). HVEM functions as a receptor for BTLA, CD160, LIGHT/TNFSF14, and Lymphotoxin-alpha (3, 8-11). Ligation of HVEM by LIGHT triggers T cell, monocyte, and neutrophil activation (7, 9) and contributes to Th1 inflammation and cardiac allograft rejection (12, 13). In contrast, HVEM binding to CD160 or BTLA suppresses T cell and dendritic cell activation (3, 6, 8, 9) and dampens intestinal inflammation (14). HVEM enhances the development of CD8+ T cell memory and Treg function (4, 5). It is additionally expressed on intestinal epithelial cells, where its binding by intraepithelial lymphocyte (IEL) expressed CD160 promotes epitheilal integrity and host defense (15). The herpesvirus envelope glycoprotein gD, which binds HVEM to initiate membrane fusion, can antagonize both BTLA and LIGHT binding (Montgomery, 8, 10, 16).

References

  1. del Rio, M.L. et al. (2010) J. Leukoc. Biol. 87:223.
  2. Hsu, H. et al. (1997) J. Biol. Chem. 272:13471.
  3. Sedy, J. R. et al. (2005) Nat. Immunol. 6:90.
  4. Tao, R. et al. (2008) J. Immunol. 180:6649.
  5. Steinberg, M.W. et al. (2013) PLoS One 8:e77992.
  6. de Trez, C. et al. (2008) J. Immunol. 180:238.
  7. Heo, S.K. et al. (2006) J. Leukoc. Biol. 79:330.
  8. Gonzalez, L.C. et al. (2005) Proc. Natl. Acad Sci. USA 102:1116.
  9. Cai, G. et al. (2008) Nat. Immunol. 9:176.
  10. Mauri, D.N. et al. (1998) Immunity 8:21.
  11. Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548.
  12. Wang, J. et al. (2005) J. Immunol. 174:8173.
  13. Ye, Q. et al. (2002) J. Exp. Med. 195:795.
  14. Steinberg, M.W. et al. (2008) J. Exp. Med. 205:1463.
  15. Shui, J.W. et al. (2012) Nature 488:222.
  16. Montgomery, R.I. et al. (1996) Cell 87:427.

Long Name

Herpesvirus Entry Mediator

Alternate Names

ATAR, CD270, LIGHTR, TNFRSF14

Entrez Gene IDs

8764 (Human); 230979 (Mouse); 102137807 (Cynomolgus Monkey)

Gene Symbol

TNFRSF14

UniProt

NP_849262 (Mouse)

Additional HVEM/TNFRSF14 Products

Product Documents for Recombinant Mouse HVEM/TNFRSF14 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse HVEM/TNFRSF14 Fc Chimera Protein, CF

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