Recombinant Human Chordin-Like 2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9127-CH

R&D Systems, part of Bio-Techne
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9127-CH-050
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Key Product Details

Source

NS0

Accession #

Q6WN34

Conjugate

Unconjugated

Applications

Bioactivity

View all Chordin-like 2/CHRDL2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Chordin-like 2/CHRDL2 protein
Leu21-Thr429, with a C-terminal 6-His tag

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu21

Predicted Molecular Mass

46 kDa

SDS-PAGE

44-65 kDa, reducing conditions

Activity

Measured by its ability to inhibit BMP-4-induced activity in MC3T3-E1 mouse preosteoblast cells.
The ED50 for this effect is 0.4-2.4 µg/mL

Formulation, Preparation and Storage

9127-CH
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution
Reconstitute at 500 μg/mL in 4 mM HCl.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chordin-like 2/CHRDL2

Chordin-like 2 (CHL2, CHRDL2, or BNF-1), is an approximately 55 kDa secreted glycoprotein that acts as a BMP antagonist. Mature human CHL2 contains three von Willebrand type C repeats and shares 73% amino acid sequence identity with mouse and rat CHL2 (1-3). Complex alternative splicing of human Chordin-like 2 generates multiple short isoforms as well as a nearly full length isoform with a substituted C-terminus (3). CHL2 is expressed in chondrocytes in developing joint cartilage (2, 4), osteoblasts, myotubes, brain, some carcinomas, and the genitourinary system (e.g. uterus, prostate, testis, bladder) (1-3). CHL2 binds to multiple TGF-beta superfamily proteins including BMP-2, -4, -5, -6, -7, GDF-5, Activin A, and TSG (2, 3, 5, 6). It prevents the binding of BMPs to type 1 and type 2 receptors as well as BMP-induced cellular responses (2, 5). CHL2 reduces the rate of matrix deposition by mesenchymal cells, acting as a negative regulator of cartilage formation (2).

References

  1. Wu, I. and M.A. Moses (2003) Gene 311:105.
  2. Nakayama, N. et al. (2004) Development 131:229.
  3. Oren, A. et al. (2004) Gene 331:17.
  4. Lorda-Diez, C.I. et al. (2013) PLoS One 8:e60423.
  5. Fujisawa, T. et al. (2009) Biochem. Biophys. Res. Commun. 385:215.
  6. Zhang, J.L. et al. (2007) J. Biol. Chem. 282:20002.

Alternate Names

BNF-1, Chordin like 2, CHRDL2

Entrez Gene IDs

25884 (Human); 69121 (Mouse)

Gene Symbol

CHRDL2

UniProt

Q6WN34

Additional Chordin-like 2/CHRDL2 Products

Product Documents for Recombinant Human Chordin-Like 2 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Chordin-Like 2 Protein, CF

For research use only

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