Recombinant Human CLEC3B/Tetranectin Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5170-CL

R&D Systems, part of Bio-Techne
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5170-CL-050
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Key Product Details

Source

NS0

Accession #

P05452

Conjugate

Unconjugated

Applications

Bioactivity

View all CLEC3B/Tetranectin Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CLEC3B/Tetranectin protein
Glu22-Val202, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu22

Predicted Molecular Mass

21.0 kDa (monomer)

SDS-PAGE

25 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.

When rhHGF (Catalog # 294-HGN) is immobilized at 5 µg/mL, the concentration of rhCLEC3B that produces 50% of the optimal binding response is found to be approximately 1-5 μg/mL.

Formulation, Preparation and Storage

5170-CL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC3B/Tetranectin

CLEC3B (C‑type lectin domain family 3 member B), also known as Tetranectin, is a 17 kDa O‑glycosylated member of the C‑type lectin superfamily (1, 2). Mature human CLEC3B consists of an alpha-helical coiled-coil region followed by one C‑type lectin domain (CTLD) (3, 4). It shares 81% amino acid sequence identity with mouse and rat CLEC3B. CLEC3B associates into non‑covalent homotrimers, although it was named Tetranectin based on the proposal that it formed tetramers (4, 5). CLEC3B is secreted by endocrine, epithelial, endothelial, and mesenchymal cells including several hematopoietic cell types (6, 7). It shows binding selectivity for heparan sulfate, fucoidan, and chondroitin sulfates A, B, and C (8). CLEC3B binds the Kringle domain-containing proteins Plasminogen, tPA, and HGF, and it enhances the tPA-mediated activation of Plasminogen (5, 9). It also reduces the ability of Angiostatin (a Plasminogen fragment) to inhibit vascular endothelial cell proliferation (10). In mouse, CLEC3B is involved in the development and repair of muscle, spine, and skin (11 - 13). CLEC3B is upregulated in stromal cells surrounding various tumors but not in the tumor cells themselves (3, 14 ‑ 16). It is concentrated in the extracellular matrix at the leading edge of malignant tumors, a pattern that overlaps that of Plasminogen (3, 14, 16).

References

  1. Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
  2. Jaquinod, M. et al. (1999) Biol. Chem. 380:1307.
  3. Wewer, U.M. and R. Albrechtsen (1992) Lab. Invest. 67:253.
  4. Nielsen, B.B. et al. (1997) FEBS Lett. 412:388.
  5. Clemmensen, I. et al. (1986) Eur. J. Biochem. 156:327.
  6. Christensen, L. and I. Clemmensen (1989) Histochemistry 92:29.
  7. Christensen, L. et al. (1987) Histochemistry 87:195.
  8. Clemmensen, I. (1989) Scand. J. Lab. Invest. 49:719.
  9. Westergaard, U.B. et al. (2003) Eur. J. Biochem. 270:1850.
  10. Mogues, T. et al. (2004) J. Biomed. Biotechnol. 2:73.
  11. Wewer, U. M. et al. (1998) Dev. Biol. 200:247.
  12. Iba, K. et al. (2001) Mol. Cell. Biol. 21:7817.
  13. Iba, K. et al. (2009) Wound Repair Regen. 17:108.
  14. Obrist, P. et al. (2004) J. Clin. Pathol. 57:417.
  15. Brunner, A. et al. (2007) Virchows Arch. 450:659.
  16. De Vries, T.J. et al. (1996) J. Pathol. 179:260.

Long Name

C-type Lectin-like Receptor 3B

Alternate Names

TETN, Tetranectin, TNA

Entrez Gene IDs

7123 (Human); 21922 (Mouse)

Gene Symbol

CLEC3B

UniProt

P05452

Additional CLEC3B/Tetranectin Products

Product Documents for Recombinant Human CLEC3B/Tetranectin Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human CLEC3B/Tetranectin Protein, CF

For research use only

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