Recombinant Human FGF-9 (HEK293-expressed) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11233-F9

R&D Systems, part of Bio-Techne
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11233-F9-050
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Key Product Details

Source

HEK293

Accession #

P31371.3

Conjugate

Unconjugated

Applications

Bioactivity

View all FGF-9 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human FGF-9 protein
Ala2-Ser208 & Leu4-Ser208

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala2 & Leu4

Predicted Molecular Mass

23 kDa

SDS-PAGE

23-30 kDa, under reducing conditions.

Activity

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 1.00-5.00 ng/mL.

Scientific Data Images for Recombinant Human FGF-9 (HEK293-expressed) Protein, CF

Recombinant Human FGF-9 (HEK293-expressed) Protein Bioactivity.

Measured in a cell proliferation assay using Balb/3T3 Mouse Embryonic Fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 1.00-5.00 ng/mL.

Recombinant Human FGF-9 (HEK293-expressed) Protein SDS-PAGE.

2 μg/lane of Recombinant Human FGF-9 (HEK293-expressed) Protein (Catalog # 11233-F9) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 23-30 kDa.

Formulation, Preparation and Storage

11233-F9
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and EDTA with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in sterile water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-9

Fibroblast growth factor 9 (FGF-9), also known as HBGF9 and GAF, is a member of the FGF family of secreted glycoproteins involved in mammalian skeleton morphogenesis and growth (1). The FGF family is characterized by a core heparin-binding FGF domain of approximately 120 amino acids (aa) that exhibits a beta -trefoil structure (2). Mature mouse FGF-9 shares 99% and 100% aa sequence identity with human and rat FGF-9, respectively. FGF-9, along with FGF-16 and -20, form a FGF subfamily that shares 65-71% aa sequence identity, binds FGFR3(IIIb), and are efficiently secreted despite having an uncleavable, bipartite signal sequence (2-4). In addition to FGFR3(IIIb), FGF-9 binding to the IIIc splice forms of FGFR-1, -2 and -3 have been reported (1,4,6). In the mouse embryo, the location and timing of FGF-9 expression affects development of the skeleton, cerebellum, lungs, heart, vasculature, digestive tract, and testes (2, 6-11). Deletion of mouse FGF-9 is lethal at birth due to lung hypoplasia, and causes rhizomelia, or shortening of the proximal skeleton (2,10,11). Additionally, a mutation in mouse FGF-9 is responsible for Elbow knee synostosis (Eks), which causes joint fusions in the elbow and knee (6). In humans, FGF9 mutations that lower receptor binding can result multiple synostoses syndrome (SYNS) (7). Altered FGF-9 expression or function is reported in human colon, endometrial, and ovarian cancers, correlating with progression, invasiveness, and survival (12-15).

References

  1. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  2. Itoh, N. and D.M. Ornitz (2008) Dev. Dyn. 237:18.
  3. Miyamoto, M. et al. (1993) Mol. Cell. Biol. 13:4251.
  4. Santos-Ocampo, S. et al. (1996) J. Biol. Chem. 271:1726.
  5. Plotnikov, A.N. et al. (2001) J. Biol. Chem. 276:4322.
  6. Harada, M. et al. (2009) Nat. Genet. 41:289.
  7. Wu, X.L. et al. (2009) Am. J. Hum. Genet. 85:53.
  8. Colvin, J.S. et al. (1999) Dev. Dyn. 216:72.
  9. Lin, Y. et al. (2009) Dev. Biol. 329:44.
  10. Hung, I.H. et al. (2007) Dev. Biol. 307:300.
  11. Colvin, J.S. et al. (2001) Dev. Dyn 128:2095.
  12. Krejci, P. et al. (2009) Hum. Mutat. 30:1245.
  13. Leushacke, M. et al. (2011) PLoS ONE 6:e23381.
  14. Hendrix, N.D. et al. (2006) Cancer Res. 66:1354.
  15. Abdel-Rahman, W.M. et al. (2008) Hum. Mutat. 29:390.

Long Name

Fibroblast Growth Factor 9

Alternate Names

FGF9

Entrez Gene IDs

2254 (Human); 14180 (Mouse)

Gene Symbol

FGF9

UniProt

P31371.3

Additional FGF-9 Products

Product Documents for Recombinant Human FGF-9 (HEK293-expressed) Protein, CF

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Product Specific Notices for Recombinant Human FGF-9 (HEK293-expressed) Protein, CF

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