Recombinant Human FGF basic Heat Stable Protein, Animal-Free Best Seller
R&D Systems, part of Bio-Techne | Catalog # BT-FGFBHS-AFL
Key Product Details
Learn more about Animal-Free Recombinant Proteins
Product Specifications
Source
E. coli-derived human FGF basic/FGF2/bFGF protein
Ala135-Ser288 (with modifications)
Produced using non-animal reagents in an animal-free laboratory.
Ala135-Ser288 (with modifications)
Produced using non-animal reagents in an animal-free laboratory.
Purity
>95%, by SDS-PAGE with quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala135 & Ala136
Predicted Molecular Mass
17 kDa
SDS-PAGE
16-19 kDa, under reducing conditions.
Activity
Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749.
The ED50 for this effect is 0.0500-0.600 ng/mL.
The ED50 for this effect is 0.0500-0.600 ng/mL.
The specific activity of Animal-Free™ Recombinant Human FGF basic Heat Stable is >1.60 x 106 IU/mg, which is calibrated against the human FGF basic/FGF2 WHO International Standard (NIBSC code: 90/712).
Scientific Data Images for Recombinant Human FGF basic Heat Stable Protein, Animal-Free
Equivalent Bioactivity of GMP, Animal-Free, and RUO grades of Recombinant Human FGF basic Heat Stable proteins.
Equivalent bioactivity of GMP (BT-FGFBHS-GMP), Animal-Free (Catalog # BT-FGFBHS-AFL) and RUO (BT-FGFBHS) grades of Recombinant Human FGF basic Heat Stable proteins as measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells (orange, green, red, respectively).
Animal-Free™ Recombinant Human FGF basic Heat Stable Bioactivity.
Animal-Free™ Recombinant Human FGF basic Heat Stable induces NR6R 3T3 mouse fibroblast cell proliferation. (A) Animal-Free™ Recombinant Human FGF basic Heat Stable (Catalog # BT-FGFBHS-AFL) or (B) wild type (WT) Recombinant Human FGF basic/FGF2/bFGF Proteins were untreated or incubated at 37°C for 3 days in media. Animal-Free™ Recombinant Human FGF basic Heat Stable protein retained similar activity to the untreated Heat Stable protein indicating that the Heat Stable protein has increased thermal stability. In contrast, WT Recombinant Human FGF basic/FGF2/bFGF Protein significantly lost activity suggesting less thermal stability.
Higher iPSC fold expansion is observed with rhFGFb Heat Stable compared to Wild Type for daily and reduced feeding at 40 ng/mL
Human iPSCs were cultured for four passages in a commercial iPSC Expansion media containing 40 ng/mL of either rhFGFb Heat Stable (BT-FGFBHS) or wild type rhFGFb, Animal-Free (BT-FGFB-AFL). Media exchanges were performed following two feeding schedules. A). a daily feed schedule or B). a reduced feed schedule, where cells were double fed twice per week. Cells were plated at 5000 cells/cm2 in a 6-well dish coated with Cultrex Ultimatrix (BME001). For each schedule, all conditions were passaged once either group reached ~60-70% confluency and counts were performed using a Cellaca™ High-Throughput Cell Counter. Count of each passage is shown comparing WT vs HS FGFb in both the A) daily feed or B) reduced feed schedule.Formulation, Preparation and Storage
BT-FGFBHS-AFL
| Formulation | Lyophilized from a 0.2 μm filtered solution in HEPES and Sodium Sulfate with Trehalose. |
| Reconstitution | Reconstitute the 25 μg size at 250 μg/mL in sterile water. Reconstitute all other sizes at 500 μg/mL in sterile water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: FGF basic/FGF2/bFGF
Our Heat Stable FGF basic has been engineered for thermostability and maintains its activity at 37℃ when compared to wild-type. Heat-stable FGF basic can enhance the flexibility and efficiency of cell culture by reducing the need for frequent media changes.
Long Name
Fibroblast Growth Factor basic
Alternate Names
bFGF, FGF-2, FGF2, HBGF-2, Prostatropin
Gene Symbol
FGF2
UniProt
Additional FGF basic/FGF2/bFGF Products
Product Documents for Recombinant Human FGF basic Heat Stable Protein, Animal-Free
Manufacturing Specifications
Animal-Free Manufacturing ConditionsOur dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.
Production
- All molecular biology procedures use animal-free media and dedicated labware.
- Dedicated fermentors are utilized in committed animal-free areas.
Purification
- Protein purification columns are animal-free.
- Bulk proteins are filtered using animal-free filters.
- Purified proteins are stored in animal-free containers in a dedicated cold storage room.
Quality Assurance
- Low Endotoxin Level.
- No impairment of biological activity.
- High quality product obtained under stringent conditions.
- For ex vivo research or bioproduction, additional documentation can be provided.
Product Specific Notices for Recombinant Human FGF basic Heat Stable Protein, Animal-Free
Produced under license from Enantis s.r.o.
For research use or further manufacturing only
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