Recombinant Human FGF basic Heat Stable Protein, CF Best Seller
R&D Systems, part of Bio-Techne | Catalog # BT-FGFBHS
Key Product Details
Product Specifications
Source
E. coli-derived human FGF basic/FGF2/bFGF protein
Ala135-Ser288 (with modifications)
Ala135-Ser288 (with modifications)
Purity
>95%, by SDS-PAGE with quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala135 & Ala136
Predicted Molecular Mass
17 kDa
SDS-PAGE
16-19 kDa, under reducing conditions.
Activity
Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749.
The ED50 for this effect is 0.0500-0.600 ng/mL.
The ED50 for this effect is 0.0500-0.600 ng/mL.
Scientific Data Images for Recombinant Human FGF basic Heat Stable Protein, CF
Equivalent Bioactivity of GMP, Animal-Free, and RUO grades of Recombinant Human FGF basic Heat Stable proteins.
Equivalent bioactivity of GMP (BT-FGFBHS-GMP), Animal-Free (BT-FGFBHS-AFL) and RUO (Catalog # BT-FGFBHS) grades of Recombinant Human FGF basic Heat Stable proteins as measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells (orange, green, red, respectively).
Recombinant Human FGF basic Heat Stable Protein, CF Bioactivity.
Recombinant Human FGF basic Heat Stable Protein induces NR6R 3T3 mouse fibroblast cell proliferation. (A) Recombinant Human FGF basic Heat Stable Protein (Catalog # BT-FGFBHS) or (B) wild type (WT) Recombinant Human FGF basic/FGF2/bFGF Proteins were untreated or incubated at 37°C for 3 days in media. Human FGF basic Heat Stable (HS) retained similar activity to the untreated HS protein indicating that the HS protein has increased thermal stability. In contrast, WT Recombinant Human FGF basic/FGF2/bFGF Protein significantly lost activity suggesting less thermal stability.
Recombinant Human FGF basic Heat Stable Protein SDS-PAGE.
2 μg/lane of Recombinant Human FGF basic Heat Stable Protein (Catalog # BT-FGFBHS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 16-19 kDa, under reducing conditions.Formulation, Preparation and Storage
BT-FGFBHS
| Formulation | Lyophilized from a 0.2 μm filtered solution in HEPES and Sodium Sulfate with Trehalose. |
| Reconstitution | Reconstitute 10 μg size at 100 μg/mL in sterile deionized water. Reconstitute all the other sizes at 500 μg/mL in sterile deionized water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: FGF basic/FGF2/bFGF
Our Heat Stable FGF basic has been engineered for thermostability and maintains its activity at 37℃ when compared to wild-type. Heat-stable FGF basic can enhance the flexibility and efficiency of cell culture by reducing the need for frequent media changes.
Long Name
Fibroblast Growth Factor basic
Alternate Names
bFGF, FGF-2, FGF2, HBGF-2, Prostatropin
Gene Symbol
FGF2
UniProt
Additional FGF basic/FGF2/bFGF Products
Product Documents for Recombinant Human FGF basic Heat Stable Protein, CF
Product Specific Notices for Recombinant Human FGF basic Heat Stable Protein, CF
Produced under license from Enantis s.r.o.
For research use only
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