Recombinant Human Fibrillin-1/FBN1 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10224-FI

R&D Systems, part of Bio-Techne
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10224-FI-050
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Key Product Details

Source

NS0

Accession #

P35555

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Fibrillin-1/FBN1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Fibrillin-1/FBN1 protein
Human Fibrillin-1
(Ala25-Thr660)
Accession # P35555		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala25

Predicted Molecular Mass

95 kDa

SDS-PAGE

97-108 kDa

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human MFAP4 (Catalog # 10230-MF) is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Human Fibrillin-1/FBN1 Fc Chimera (Catalog # 10224-FI)  that produces 50% of the optimal binding response is 0.1-0.6 μg/mL.

Scientific Data Images for Recombinant Human Fibrillin-1/FBN1 Fc Chimera Protein, CF

Recombinant Human Fibrillin-1/FBN1 Fc Chimera Protein Binding Activity

Recombinant Human Fibrillin-1/FBN1 Fc Chimera Protein Binding Activity

When Recombinant Human MFAP4 (Catalog # 10230-MF) is coated at 0.5 µg/mL, 100 µL/well, Recombinant Human Fibrillin-1/FBN1 Fc Chimera (Catalog # 10224-FI) binds with an ED50 of 0.1-0.6 µg/mL.
Recombinant Human Fibrillin-1/FBN1 Fc Chimera Protein SDS-PAGE

Recombinant Human Fibrillin-1/FBN1 Fc Chimera Protein SDS-PAGE

1 μg/lane of Recombinant Human Fibrillin-1/FBN1 Fc Chimera (Catalog # 10224-FI) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 97-108 kDa and 190-220 kDa, respectively.

Formulation, Preparation and Storage

10224-FI
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fibrillin-1/FBN1

Fibrillins are glycoproteins forming the backbone of microfibrils in elastic and non-elastic tissues. They interact with other components of the extracellular matrix (ECM) and play essential roles in tissue development, homeostasis and repair. Fibrillin-1 is a calcium-binding protein that assembles to form the structural component of the 10-12 nm microfibrils of the ECM. The human Fibrillin-1 has multiple domains, primarily consisting of epidermal growth factor (EGF)-like and other modules (1, 2). The calcium-binding modules in some of the EGF domains provide structural stability and the characteristic rod-like shape of the protein (3-8). Mature human Fibrillin-1 shares 97% amino acid (aa) sequence identity with mature mouse Fibrillin-1. Human Fibrillin-1 is synthesized as an approximately 350-kDa precursor molecule, which is then proteolytically processed by furin into its biologically active form (9-10). Fibrillin microfibers are further engaged in a number of cell matrix interactions such as with integrins, bone morphogenetic proteins (BMPs) and the large latent complex of transforming growth factor-beta (11). Fibrillin-1 mutations are associated with a range of heritable connective disorders, including Marfan syndrome and acromelic dysplasias (11-12).

References

  1. Robertson, I. et al. (2011) Biochem. J. 433:263.
  2. Corson, G.M. et al. (1993) Genomics 17:476.
  3. Maslen, C.L. et al. (1991) Nature 352:334.
  4. Hanford, P.A. et al. (1991) Nature 353:395.
  5. Werner, J.M. et al. (2000) J. Mol. Biol. 296:1065.
  6. Downing, A.K. et al. (1996) Cell 85:597.
  7. Smallridge, R.S. et al. (2003) J. Biol. Chem. 278:12199.
  8. Reinhardt, D.P. et al. (1997) J. Biol. Chem. 272:7368.
  9. Raghunath, M. et al. (1999) J. Cell. Sci. 112:1093.
  10. Wallis, D.D. et al. (2003) J. Cell. Sci. 90:641.
  11. Jensen, S.A. et al. (2016) Biochem. J. 473:827.
  12. Sherratt, M.J. et al. (2001) Micron. 32:185.

Long Name

Fibrillin-1

Alternate Names

ACMICD, Asprosin, Fibrillin 1, GPHYSD2, SSKS, WMS2

Entrez Gene IDs

2200 (Human)

Gene Symbol

FBN1

UniProt

P35555

Additional Fibrillin-1/FBN1 Products

Product Documents for Recombinant Human Fibrillin-1/FBN1 Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human Fibrillin-1/FBN1 Fc Chimera Protein, CF

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