Recombinant Human MFAP4 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 10230-MF
Key Product Details
Source
CHO
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human MFAP4 protein
Val22-Ala255, with an N-terminal c-Myc Tag
Val22-Ala255, with an N-terminal c-Myc Tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Glu (c-Myc Tag)
Predicted Molecular Mass
28 kDa
SDS-PAGE
35-45 kDa, under reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
When Recombinant Human MFAP4 (Catalog # 10230-MF) is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Human Fibrillin-1/FBN1 Fc Chimera (Catalog # 10224-FI) nbsp; that produces 50% of the optimal binding response is 0.1-0.6 μg/mL.
When Recombinant Human MFAP4 (Catalog # 10230-MF) is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Human Fibrillin-1/FBN1 Fc Chimera (Catalog # 10224-FI) nbsp; that produces 50% of the optimal binding response is 0.1-0.6 μg/mL.
Scientific Data Images for Recombinant Human MFAP4 Protein, CF
Recombinant Human MFAP4 Protein Binding Activity
When Recombinant Human MFAP4 (Catalog # 10230-MF) is coated at 0.5 µg/mL, 100 µL/well, Recombinant Human Fibrillin-1/FBN1 Fc Chimera (Catalog # 10224-FI) binds with an ED50 of 0.1-0.6 µg/mL.Recombinant Human MFAP4 Protein SDS-PAGE
2 μg/lane of Recombinant Human MFAP4 (Catalog # 10230-MF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 35-45 kDa and 70-90 kDa, respectively.Formulation, Preparation and Storage
10230-MF
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Reconstitution | Reconstitute at 500 μg/mL in PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: MFAP4
References
- Pilecki, B. et al. (2016) J. Biol. Chem. 291:1103.
- Gressner, O.A. et al. (2007) J. Cell Mol. Med. 11:1031.
- Schlosser A. et al. (2006) Scand J Immunol. 64:104.
- Hunes RO. (1992) Cell. 69:11.
- Kasamatsu, S. et al. (2011) Sci. Rep. 1:164.
- Lausen, M. et al. (2017) J. Biol. Chem. 274:32234.
- Molleken, C. et al. (2009) Hepatology. 49:1257.
Long Name
Microfibril-associated Glycoprotein 4
Alternate Names
MFAP-4
Gene Symbol
MFAP4
UniProt
Additional MFAP4 Products
Product Documents for Recombinant Human MFAP4 Protein, CF
Product Specific Notices for Recombinant Human MFAP4 Protein, CF
For research use only
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