Recombinant Human GDF-5 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8340-G5

R&D Systems, part of Bio-Techne
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8340-G5-050
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Key Product Details

Source

E. coli

Accession #

P43026

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all GDF-5/BMP-14 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human GDF-5/BMP-14 protein
Ala382-Arg501

Purity

>90%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala382 and Pro383

Predicted Molecular Mass

14 kDa

SDS-PAGE

14 kDa, reducing conditions

Activity

Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351.
The ED50 for this effect is 0.2-1.2 μg/mL.

Scientific Data Images for Recombinant Human GDF-5 Protein, CF

Recombinant Human GDF-5 Protein Bioactivity

Recombinant Human GDF-5 Protein Bioactivity

Recombinant Human GDF-5 (Catalog # 8340-G5) induces alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.2-1.2 μg/mL.

Formulation, Preparation and Storage

8340-G5
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution
Reconstitute at 250 μg/mL in 4 mM HCl.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-5/BMP-14

Growth Differentiation Factor-5 (GDF-5; also called BMP-14 and CDMP-1) is a member of the BMP family of TGF-beta superfamily proteins (1, 2). Human GDF-5, -6, and -7 are a defined subgroup of the BMP family (3). GDF-5 is synthesized as a homodimeric precursor protein consisting of a 354 amino acid (aa) N-terminal pro-region and a 120 aa C-terminal mature peptide. Mature human GDF-5 shares 99% aa sequence identity with both mature mouse and rat GDF-5. GDF-5 signaling is mediated by formation of a heterodimeric complex consisting of a type 1 (BMPR-IB) and a type II (BMPR-II or Activin RII) serine/threonine kinase receptor which results in the phosphorylation and activation of cytosolic Smad proteins (Smad1, 5, and 8) (4, 5). Similar to other BMP family proteins, GDF-5 signaling is antagonized by Noggin (6). GDF-5 is involved in multiple developmental processes including limb generation, cartilage development, joint formation, bone morphogenesis, cell survival, and neuritogenesis (7-11). Exogenous GDF-5 has been reported to promote chondrogenesis, osteogenesis, and angiogenesis in mesenchymal stem cells in vivo and in vitro (12-14). Inhibition of GDF-5 expression or alteration of its signaling can facilitate the development of osteoarthritis (15-18).

References

  1. Storm, E.E. et al. (1994) Nature 368:639.
  2. Hotten, G. et al. (1994) Biochem. Biophys. Res. Commun. 204:646.
  3. Mikic, B. (2004) Ann. Biomed. Eng. 32:466.
  4. Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
  5. Mueller, T.D. and J. Nickel (2012) FEBS Lett. 586:1846.
  6. Bragdon, B. et al. (2011) Cell. Signal. 23:609.
  7. Sieber, C. et al. (2006) Biol. Chem. 387:451.
  8. Osorio, C. et al. (2013) Development 140:4751.
  9. Wood, T.K. et al. (2005) J. Neurosci. Res. 80:759.
  10. Francis-West, P.H. et al. (1999) Development 126:1305.
  11. Brunet, L.J. et al. (1998) Science 280:1455.
  12. Zeng, Q. et al. (2007) Bone 40:374.
  13. Coleman, C.M. et al. (2013) Stem Cells Dev. 22:1968.
  14. Cheng, X. et al. (2012) Cells Tissues Organs 196:56.
  15. Zhang, Y. et al. (2014) Exp. Mol. Med. 46:e79.
  16. Miyamoto, Y. et al. (2007) Nat. Genet. 39:529.
  17. Vaes, R.B. et al. (2009) Ann. Rheum. Dis. 68:1754.
  18. Degenkolbe, E. et al. (2013) PLoS Genet. 9:e1003846.

Long Name

Growth Differentiation Factor 5

Alternate Names

BMP-14, CDMP-1, GDF5

Entrez Gene IDs

8200 (Human); 14563 (Mouse)

Gene Symbol

GDF5

UniProt

P43026

Additional GDF-5/BMP-14 Products

Product Documents for Recombinant Human GDF-5 Protein, CF

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Product Specific Notices for Recombinant Human GDF-5 Protein, CF

For research use only

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