Recombinant Human GDNF, Animal Free Protein
R&D Systems, part of Bio-Techne | Catalog # Qk051
Key Product Details
Product Specifications
Source
E. coli-derived human GDNF protein
Purity
Single species with expected mass
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Predicted Molecular Mass
30.4 kDa (dimer), 15 kDa (monomer)
SDS-PAGE
Dimeric GDNF protein only
Activity
No significant difference between EC50 of reference and test lots
Mycoplasma
Negative when tested in both ribosomal RNA hybridization and luminescence assays
Scientific Data Images for Recombinant Human GDNF, Animal Free Protein
Recombinant Human GDNF, Animal Free Protein Bioactivity
GDNF activity is determined using an SH-SY5Y cell proliferation assay. Cells were incubated in duplicates with different concentrations of GDNF in the presence of retinoic acid and recombinant GFR alpha1 for 3 days before viable cell measurement using an MTS assay. Cell proliferation is measured and normalized.EC50 = 17.5 ng/ml (0.58 nM).
Recombinant Human GDNF, Animal Free Protein SDS-PAGE
GDNF migrates as a single band at 30 kDa in non-reducing (NR) conditions and 15 kDa upon reduction (R). No contaminating protein bands are visible.Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+ beta-mercaptoethanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250.Formulation, Preparation and Storage
Qk051
| Formulation | Lyophilized from acetonitrile/TFA |
| Reconstitution | Resuspend in water at >100 µg/ml, prepare single use aliquots, add carrier protein if desired. |
| Shipping | The product is shipped lyophilized at ambient temperture, on ice blocks or dry ice. Shipping at ambient temperture does not affect the bioactivity or stability of the protein. Upon reciept, store immediately at the conditions stated below. |
| Stability & Storage | Store lyophilized protein between -20 and -80 °C until the date of expiry. Avoid freeze-thaw cycles. |
Background: GDNF
Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. NS0 expressed mature human GDNF lacks 31 residues from the amino-terminus of the predicted sequence. This glycosylated recombinant mature human GDNF still contains the seven conserved Cys residues found in all members of the TGF-beta superfamily and is biologically active. The GDNF sequence contains two potential glycosylation sites and insect cell‑expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.
Long Name
Glial Cell line-derived Growth Factor
Alternate Names
ATF, HFB1-GDNF, HGDNF, HSCR3
Gene Symbol
GDNF
UniProt
Additional GDNF Products
Product Documents for Recombinant Human GDNF, Animal Free Protein
Product Specific Notices for Recombinant Human GDNF, Animal Free Protein
The above product was manufactured, tested and released by R&D System's contract manufacturer, Qkine Ltd, at 1 Murdoch House, Cambridge, UK, CB5 8HW. The product is for research use only and not for the diagnostic or theraputic use.
For research use only
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